Expression Vectors

CSB plasmids are freely available to Vanderbilt researchers. Contact Noah Saracho

for more information. The links for ‘Single Gene Expression’ require login with VUNet ID and password.

Single Gene Expression in E. coli:

Multiple Gene Expression:

• Duet vectors for E. coli expression
• MultiColi vectors for E. coli expression
• MultiBac vectors for baculovirus expression

Membrane/Toxic Protein Expression

Recombinant Protease Expression:

• 3C protease
• TEV protease
• Ulp1 (Sumo Protease)

Protocols:

• Sequence and Ligation Independent Cloning (SLIC) protocol
• Megawhop protocol for multi-site mutagenesis

Sequencing Primers