Expression Vectors
CSB plasmids are freely available to Vanderbilt researchers. Contact Alex Fisch for more information. The links below require login with VUNet ID and password.
Single Gene Expression in E. coli:
NT Tag(s) | Protease Cleavage Site | CT Tag(s) | Resistance | Vector |
---|---|---|---|---|
GST | TEV | His8 | Kan | pAT110 |
3C/PreScission | His8 | Kan | pAT109 | |
His6 | Thrombin | HSV•His6 | Kan | pSV277 |
TEV | HSV•His6 | Kan | pSV281 | |
3C/PreScission | HSV•His6 | Kan | pBG100* | |
His6 | Amp | pHD116* | ||
His10 | Thrombin | His8 | Kan | pNR114* |
TEV | His8 | Kan | pNR111* | |
3C/PreScission | His8 | Kan | pBG106* | |
His•GST | TEV | HSV•His6 | Kan | pBG105* |
3C/PreScission | HSV•His6 | Kan | pBG101* | |
His•MBP | Thrombin | HSV•His6 | Kan | pSV278* |
TEV | HSV•His6 | Kan | pSV282* | |
3C/PreScission | HSV•His6 | Kan | pLM302* pLM303* |
|
His•SUMO | TEV | HSV•His6 | Kan | pHD115* |
3C/PreScission | HSV•His6 | Kan | pBG102* pBG103* |
|
Ulp1 | HSV•His6 | Kan | pBG104* | |
MBP | TEV | His8 | Kan | pAT108 |
3C/PreScission | His8 | Kan | pAT107 | |
Trx•His | TEV | His6 | Amp | pNR113 |
3C/PreScission | HSV•His6 | Kan | pLM304 | |
His6 | Amp | pNR112 |
*An anti-His antibody to the HHHHHHGSS sequence encoded by this vector is sold through the Molecular Biology Core.
Multiple Gene Expression:
- Duet vectors for E. coli expression
- MultiColi vectors for E. coli expression
- MultiBac vectors for baculovirus expression
Membrane/Toxic Protein Expression
Recombinant Protease Expression:
Protocols:
Sequencing Primers