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Expression Vectors

CSB plasmids are freely available to Vanderbilt researchers. Contact Noah Saracho

for more information. The links for ‘Single Gene Expression’ require login with VUNet ID and password.

Single Gene Expression in E. coli:

NT Tag(s) Protease Cleavage Site CT Tag(s) Resistance Vector
GST TEV His8 Kan pAT110
3C/PreScission His8 Kan pAT109
His6 Thrombin HSV•His6 Kan pSV277
TEV HSV•His6 Kan pSV281
3C/PreScission HSV•His6 Kan pBG100
His6 Amp pHD116
His10 Thrombin His8 Kan pNR114
TEV His8 Kan pNR111
3C/PreScission His8 Kan pBG106
His•GST TEV HSV•His6 Kan pBG105
3C/PreScission HSV•His6 Kan pBG101
His•MBP Thrombin HSV•His6 Kan pSV278
TEV HSV•His6 Kan pSV282
3C/PreScission HSV•His6 Kan pLM302
His•SUMO TEV HSV•His6 Kan pHD115
3C/PreScission HSV•His6 Kan pBG102
Ulp1 HSV•His6 Kan pBG104
MBP TEV His8 Kan pAT108
3C/PreScission His8 Kan pAT107
Trx•His TEV His6 Amp pNR113
3C/PreScission HSV•His6 Kan pLM304
His6 Amp pNR112


Multiple Gene Expression:


Membrane/Toxic Protein Expression


Recombinant Protease Expression:




Sequencing Primers