Sequencing Primers
The CSB stocks most (but not all) of the following. Primers for sequencing Duet coexpression vectors are listed here.
| Use | Name | Sequence | Comments |
|---|---|---|---|
| Forward, GST fusion | GSTseq (pGEX-F) |
5′-GGGCTGGCAAGCCACGTTTGGTG | Anneals near end of GST tag in pAT109/110, pBG101/105 |
| Forward, MBP fusion | pMAL-F | 5′-GGTCGTCAGACTGTCGATGAAGCC | Anneals near end of MBP tag in pAT107/108, pLM302/303, pSV278, pSV282 |
| Forward, Sumo fusion | SUMOSeq | 5′-AGATTCTTGTACGACGGTATTAG | Anneals near end of Sumo tag in pHD115, pBG102/103/104 |
| Forward, T7 | T7 universal | 5′-TAATACGACTCACTATAGGG | For forward sequencing of all pET-based CSB vectors. |
| Forward, Trx fusion | Trx tag primer | 5′-GTGCACTGTCTAAAGGTCAG | Anneals near end of Trx tag in pLM304, pNR112/113. Novagen sells this. |
| Reverse, T7 | T7Rev | 5′-GCTAGTTATTGCTCAGCGGT | For reverse sequencing of all CSB vectors |