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Sequencing Primers

The CSB stocks most (but not all) of the following. Primers for sequencing Duet coexpression vectors are listed here.

Use Name Sequence Comments
Forward, GST fusion GSTseq
5′-GGGCTGGCAAGCCACGTTTGGTG Anneals near end of GST tag in pAT109/110, pBG101/105
Forward, MBP fusion pMAL-F 5′-GGTCGTCAGACTGTCGATGAAGCC Anneals near end of MBP tag in pAT107/108, pLM302/303, pSV278, pSV282
Forward, Sumo fusion SUMOSeq 5′-AGATTCTTGTACGACGGTATTAG Anneals near end of Sumo tag in pHD115, pBG102/103/104
Forward, T7 T7 universal 5′-TAATACGACTCACTATAGGG For forward sequencing of all pET-based CSB vectors.
Forward, Trx fusion Trx tag primer 5′-GTGCACTGTCTAAAGGTCAG Anneals near end of Trx tag in pLM304, pNR112/113. Novagen sells this.
Reverse, T7 T7Rev 5′-GCTAGTTATTGCTCAGCGGT For reverse sequencing of all CSB vectors