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pH6H3C

For expression of N-terminally His-tagged human rhinovirus 3C protease in E. coli.


General Information:

  • pET derivative
  • T7lac promoter
  • low copy
  • Kan resistance
  • dna sequence
  • 3C protease recognition sequence: LEVLFQ↓GP
  • MW = 21.8 kDa
  • Amino acid sequence:

MGSSHHHHHH GGGLVPRGPN TEFALSLLRK NIMTITTSKG EFTGLGIHDR VCVIPTHAQP GDDVLVNGQK IRVKDKYKLV DPENINLELT VLTLDRNEKF RDIRGFISED LEGVDATLVV HSNNFTNTIL EVGPVTMAGL INLSSTPTNR MIRYDYATKT GQCGGVLCAT GKIFGIHVGG NGRQGFSAQL KKQYFVEKQ

Protocol: 3C protease expression and purification — This document also contains some information on buffer components that affect protease activity.