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Center for Structural Biology

Cryo-EM Equipment

Equipment and Resources

Visit the facility iLab site for equipment reservations, training, and consultation requests.

Information to set up an iLab Account: iLab Account Basics and Setting Up a New User Account Guide.

Microscopes

  • Titan Krios G4, 300kV TEM with Gatan K3 BioQuantum and ThermoScientific Falcon3 direct electron detectors: for collecting large dataset of high-resolution cryo single particle and tomography data
  • Glacios, 200kV TEM with Falcon4i direct electron detector: for screening vitrified grids and collecting small data sets of high-resolution cryo single particle, tomography, and MicroED data
  • L120C, 100kV TEM with Ceta-S 4k x 4k CMOS detector: for screening and collecting negative stain data

Auxiliary

  • 2 ThermoScientific Vitrobots, Mark II and Mark IV
  • 1 Leica EM GP2 plunge freezer
  • 2 Gatan 626 cryo-transfer holders
  • 2 Carbon evaporators
  • 2 Glow dischargers
  • GPU workstations

Other on-campus equipment

  • Helios NanoLab G3 CX Dual Beam FIB-SEM with Quorum PP3010T Cryo System: for preparing lamella for TEM imaging and other capabilities.  See VINSE website for more info.
  • Tecnai G2 Osiris S/TEM: for imaging of purified and thin sectioned samples and EDS element detection.  See VINSE website for more info.
  • JEOL TEM 2100 Plus TEM: for imaging of purified and thin sectioned samples.  See CISR website for more info.
  • Zeiss Crossbeam 550 FIB-SEM: for preparing lamella for TEM imaging and other capabilities.  See CISR website for more info.