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Cryo-EM Links & Resources

Below are several links to serve as a starting point for the EM and Cryo-EM world, but don’t hesitate to Contact Us with questions.

 

General Information

Collaborative Computational Project for Electron cryo-Microscopy” –  Medial Research Council

Getting Started in Cryo-EM” – Lecture videos from Caltech

Beginners Guide to TEM” – by John Rodenburg

Electron Microscopy for Dummies” – public domain series

CryoEM101” – Workflow tutorial from University of Utah

MyScope TEM Introduction” – Australian Microscopy & Microanalysis Research Facility

3D Electron Microscopy (3DEM)” – WikiBooks

Lenses and Apertures of A TEM” – EMA 6518 Course Presentation by Dr. C. Wang

SBGridTV” – Videos from events hosted by SBGrid on EM, crystallography, structure analysis, computational chemistry and more

Electron Cryo-microscopy Course 2017” – Lectures from the MRC Laboratory of Molecular Biology

Official 2017 Nobel Lectures in Physics and Chemistry at Stockholm Univeristy” – Lectures on the history and scientific accomplishments in the Cryo-EM field by Richard Henderson, Jacques Dubochet, and Joachim Frank

 

Tutorials & Techniques

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM” – Jove video

Graphene Oxide Grid Preparation” – by Thomas G. Martin, Scheres Group, Laboratory of Molecular Biology, Cambridge, UK

EM-University” – Workflow videos for using Vitrobot, clipping grids, and EPU

RELION” – Wiki & tutorial

Software Tools for Molecular Microscopy” – WikiBooks

Tomography Software” – NoskeWiki

Electron Microscopy” – YouTube playlist by SBGrid Consortium

 

Meetings & Workshops

3DEM meetings and workshops” – EMDataResource

Simons Electron Microscopy Center Workshops and Courses” – New York Structural Biology Center

Workshops and Symposia at Outside Organizations” – New York Structural Biology Center

 

Databases

Unified Data Source for 3-Dimensional Electron Microscopy” – EMDataBank

Electron Microscopy Data Bank” – Protein Data Bank in Europe

Electron Microscopy Public Image Archive” – public resource for raw, 2D electron microscopy images

 

Mailing Lists

3DEM” – Information for the 3DEM Community

CCPEM” – Email discussion lists for the UK Education and Research Communities

 

Further reading for your free time

Realizing the potential of electron cryo-microscopy” – R. Henderson (2004)

A Primer to Single-Particle Cryo-Electron Microscopy” – Y. Cheng, N. Grigorieff, P. Panczek, and T. Walz (2015)

Cryo-Electron Microscopy and the Amazing Race to Atomic Resolution” – E. Binshtein and M. Ohi (2015)

Single-Particle Cryo-EM at Crystallographic Resolution” – Y. Cheng (2015)