High-Throughput Multi-Omic Analyses

In collaboration with the Vanderbilt DARPA RTA Team (R. Caprioli, E. Skaar, J. Wikswo, J. McLean, B. Lacey, J. Norris), the CIT has contributed to developing an automated multi-omic sample preparation approach. The approach, sample preparation for multi-omics technologies (SPOT), provides equivalent performance to typical individual omic preparation methods but greatly enhances throughput and minimizes the resources required for multiomic experiments. In this manuscript, SPOT was used to understand the mechanism of action of Zn-treated HL-60 cells using transcriptomic, proteomic and metabolomic data generated from a common cell culture sample.

The details are available in the Journal of Proteome Research:
https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.8b00302