Mass Spectrometry Instrumentation at the CIT

Welcome to the Center for Innovative Technology (CIT) at Vanderbilt University!

The CIT is a collaborative analytical facility specializing in a range of cutting edge mass spectrometry (MS) and confocal microscopy techniques. The Center focuses on metabolomics-based analysis and provides the infrastructure and expertise to measure both untargeted and targeted small molecules derived from biospecimens (cells, tissues, fluids, etc.). In addition, we place an emphasis on the use and development of novel separation technologies coupled to MS including hydrophobic/hydrophilic liquid chromatography (RPLC and HILIC), supercritical fluid chromatography, and ion mobility-mass spectrometry (IM-MS), all of which are supported by current state-of-the-art informatics and data analysis software. The combination of these capabilities form the basis for the analyses of diverse sample matrices such as cell cultures, blood, plasma, and urine, as well as direct effluent analysis of advanced systems such as organ-on-chip microfluidic or 3-dimensional devices.

Our shared resource core facility is part of the VU C.O.R.E.S. system.

Fluidic Valves Mass Spectrometry

Research Support

For routine applications the CIT offers the following capabilities:

  • Consultation and experimental design
  • Sample preparation and processing
  • High resolution and high mass accuracy LC-MS/MS or LC-IM-MS/MS data collection
  • High-content, confocal microscopy
  • Data processing, analysis, and basic statistical analyses
  • Data interpretation and pathway analysis

Please review our publications for additional information.

Specialized Analysis Software

We use next-generation commercial software to process and analyze LC and MS measurements from data-dependent analysis (DDA) and data independent analysis (DIA) data sets as well as targeted data acquisitions.

For data acquired via IM-MS, the software utilizes the additional dimension of ion-mobility for enhanced peak capacity and signal-to-noise ratio (S/N).

An array of statistical tools is used to prioritize features or compounds for investigation, annotation, and/or validation.

Contact Us

Please email us today to discuss your next MS-supported research project or to receive more information about the services we offer.