Targeted and Non-Targeted Global Mass Spectrometry Instrumentation Lineup at the CIT

Welcome to the Center for Innovative Technology (CIT) at Vanderbilt University!

The CIT is a collaborative analytical facility and shared resource center specializing in a range of cutting edge mass spectrometry (MS) and confocal microscopy techniques. The Center focuses on metabolomics-based analyses and provides the infrastructure and expertise to measure both untargeted and targeted small molecules derived from biospecimens (cells, tissues, biofluids, etc.). In addition, our research facility places an emphasis on the use and development of novel separation technologies coupled to MS including hydrophobic/hydrophilic liquid chromatography (RPLC and HILIC), supercritical fluid chromatography, and ion mobility-mass spectrometry (IM-MS). Further, all of these technologies are supported by current state-of-the-art informatics and data analysis software. The combination of these capabilities form the basis for the analyses of diverse sample matrices such as cell cultures, blood, plasma, and urine, as well as direct effluent analysis of advanced systems such as organ-on-chip microfluidics or 3-dimensional devices.

Our shared resource core facility is part of the Vanderbilt University iLab system.

Fluidic Valves Mass Spectrometry

Research Support

For routine applications the CIT offers the following capabilities:

  • Consultation and experimental design
  • Sample preparation and processing
  • High resolution and high mass accuracy LC-MS/MS or LC-IM-MS/MS data collection
  • High-content confocal screening microscopy
  • Data processing, analysis, and basic statistical analyses
  • Data interpretation and pathway analysis

Please review our publications for additional information.

Specialized Analysis Software

We use next-generation commercial software to process and analyze liquid chromatography (LC) and mass spectrometry (MS) measurements from data-dependent analysis (DDA) and data independent analysis (DIA) data sets as well as targeted data acquisitions.

For data acquired via ion mobility-mass spectrometry (IM-MS), the software utilizes the additional dimension of ion-mobility for enhanced peak capacity and signal-to-noise ratio (S/N).

An array of statistical tools is used to prioritize features or compounds for investigation, annotation, and/or validation.

Contact Us

Please send us an inquiry in iLab today to discuss your next metabolomics-supported research project or to receive more detailed information about the services we offer.

Our team has over 30 years of combined experience in mass spectrometry, data analysis, and informatics pipelines and we look forward to collaborating with you.