Agilent Technologies at Vanderbilt University

Agilent 6560 LC-IM-MS

Mass Spectrometry Shared Resource Collaboration

Waters Synapt G2 LC-IM-MS and
Waters Synapt G2-S LC-IM-MS (not shown)

Thermo Extractive HF

Thermo Q-Exactive HF LC-MS

  • Mass Spectrometry Technology & Instrumentation

    The CIT houses state-of-the-art mass spectrometry technology. Our instrumentation includes:

    • four commercial LC-ion mobility-Q-TOF mass spectrometers
    • a high resolution LC-Orbitrap mass spectrometer
    • a triple quadrupole LC-MS instrument

    These instruments are supported by a variety of sample handling accessories including supercritical fluid chromatography (SFC), normal and reverse-phase liquid chromatography (HILIC and RPLC), and a nanoflow ChipCube platform.

    Importantly, the CIT (in conjunction with the McLean laboratory) is designated as a Waters Center of Innovation (Waters Corporation) and recognized as an Agilent Thought Leader laboratory (Agilent Technologies).

  • High-Content Screening Technology & Instrumentation

    Another state-of-the-art resource at the CIT is the high-content confocal ArrayScan Infinity XTI (Thermo Scientific) microscope. This screening instrument is capable of simultaneous imaging and analysis of entire wellplates, high-resolution confocal or widefield imaging, and time-lapse acquisition in up to 7 different fluorescent color channels.

    Our Thermo Scientific ArrayScan microscope is a highly flexible, fully automated high-content platform that allows users to choose between widefield and confocal imaging mode, brightfield and fluorescence illumination, and live or fixed-cell experimental setups. It has a seven-color LED solid-state illumination scheme (incl. far-red and near-infrared), a CrEST X-Light spinning disk confocal module, a 14-bit Sony SuperHAD CCD camera, and various emission filter sets. The automated, environmentally-controlled stage accepts 6-well to 1536-well screening plates and the powerful, highly intuitive software package enables online analysis of entire plates with pre-stablished and fully customizable, automated analysis scripts.

  • Data Processing & Analysis Workstations

    Our Center has invested heavily in licensing to provide personnel and collaborators access to next-generation software and small molecule libraries/databases including Progenesis QI, Compound Discoverer, MassHunter and Mass Profiler, METLIN, and NIST 14 mass spectral libraries, among others. Consequently, the CIT has ten shared computer workstations for data acquisition, control, and analysis. Three of these workstations are dedicated to offline data visualization, processing, and analysis.

    For the purpose of data handling, our shared resource center has a dedicated full-time computer programmer/ software analyst to assist in streamlining data analysis, developing in-house programs to support workflows, maintaining and updating all data workstations, and automating data storage backup of all instrumentation. Complementary, the CIT houses a 24 TB RAID storage bank where all data (original and metadata) is automatically archived onto backup servers to support the breadth of CIT activities.

Mass Spectrometry Sample Vials
Shared Resource Core Workstation
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