Detection of Middle Ear Infections
The objective of this project is to develop a hand-held optical device to detect biochemical markers of wildtype, mutant and resistant strains of bacteria that cause otitis media (OM). The goal is to develop an assay that will track these biomarkers in real-time in vivo for diagnosis and monitoring of infection to inform clinical management decisions. Specifically, we seek to: (1) characterize biomarkers associated with bacteria that cause AOM using Raman microspectroscopy, (2) identify bacteria in human middle ear effusion (MEE) using Raman spectroscopy, and (3) design a Raman system to identify bacteria causing AOM in a clinical setting. Biochemical characterization of bacteria involved in ear infections will be achieved using Raman spectroscopy (RS). Clinical samples from ear infections will be evaluated to identify bacteria. A clinical Raman scanner will be developed to identify bacteria involved in ear infections.
Post Doctoral Scholars
Girish Hiremath , Vanderbilt University Medical Center
Doster, R., Ayala, O., Manning, S., Aronoff, D., Mahadevan-Jansen, A., & Gaddy, J. (2018). 2011. Identification of Streptococcus agalactiae on Human Fetal Membrane Tissues Using Raman Microspectroscopy. Open Forum Infectious Diseases , 5 (Suppl 1),
Ayala, O. D. et al. Drug-Resistant Staphylococcus aureus Strains Reveal Distinct Biochemical Features with Raman Microspectroscopy. ACS Infect. Dis. 4, 1197–1210 (2018).
Ayala, O. D. et al. Characterization of bacteria causing acute otitis media using Raman microspectroscopy. Anal. Methods 9, 1864–1871 (2017).