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Biophotonics Seminar Series

Upcoming Seminar

 Kristen Maitland

 

Presenter:

Kristen Maitland, Ph.D

 

Affiliation:

Associate Professor of Biomedical Engineering, Director of the Microscopy and Imaging Center, Texas A&M University

 

When: 

Tuesday, November 17th 12:00-1:00 PM

 

Where:

Zoom: Meeting ID: 957 8567 772,

Passcode: VBC2020

 

Seminar Title: 

Optical imaging technologies for detection of pulmonary tuberculosis

Abstract:

 

Tuberculosis afflicts one third of the population and is one of the top ten causes of death in children worldwide. Rapid diagnostics and more effective therapeutics are urgently needed. However, the slow growth rate of Mycobacterium tuberculosis, the causative agent of tuberculosis, hinders advancement in all areas of TB research. Fluorescent proteins have been widely utilized in real-time imaging of tuberculosis to track disease progression and evaluate therapeutic outcomes in preclinical models, but the sensitivity of this technique is limited by tissue absorption of fluorescence excitation light particularly in the visible wavelengths. To circumvent the impact of tissue absorption of excitation light, we have applied a fiber bundle microendoscope to deliver fluorescence excitation light directly into the mouse lung. By integrating this microendoscope into a whole animal imaging system to enable intravital excitation in the mouse lung combined with whole animal detection, we have improved the detection threshold of tdTomato expressing M. bovis BCG strain during pulmonary infection to ~103 colony forming units (CFU). This detection threshold represents a ~3-fold improvement compared to epi-illumination in a whole animal imaging system. Translating towards sensing clinical strains of M. tuberculosis, we utilize reporter enzyme fluorescence technology designed to be cleaved by the BlaC β-lactamase enzyme. Using this near-infrared fluorescent probe, we have increased sensitivity by 10x to 100 CFU mycobacteria in pulmonary infection in mice.Additionally, we developed a computer model of light transport in infected mouse lungs and three-dimensional tissue phantoms that validate the experimental results. 

 

 

 

Future Seminars (Fall 2020)

Date Time          
Location   
Name Affiliation
Nov 17 12:00-1:00pm Zoom Kristen Maitland Texas A&M University
Dec 1 4:00-5:00pm Zoom Kishan Dholakia University of St. Andrews
Dec 8 12:00-1:00pm Zoom Rita Strack Nature Methods

For up to date announcements regarding time, location, and changes to the schedule, please join the Biophotonics Seminar listserv by emailing Dr. Andrea Locke or Dr. Iftak Hussain.

Past Seminars

2020      
Cody Siciliano, PhD  David Levitz, PhD  Yevgenia Kozorovitskiy, PhD  Curtis Marcott, PhD
Irene Georgakoudi, PhD  Rebecca Vincelette, PhD  Brian Applegate, PhD  Teng-Leong Chew, PhD
Duncan Graham, PhD  Kostandinka Bizheva, PhD    
2019      
Pierre Massion, MD Justus Ndukaife, PhD Ashok Choudhury, PhD Jennifer Hunter, PhD
Christopher Valdez, PhD Mala Mahendroo, PhD Hari Schroff, PhD Maryellen Giger, PhD
Bernard Choi, PhD Richard Simerly, PhD Richard Dluhy, PhD Daniel Hammer, PhD
Shy Shoham, PhD Jennifer Hunter, PhD    
2018      
Sandra J. Rosenthal, PhD  Bryan Millis, PhD Thomas Wang, PhD, MD  Robert V. Chimenti
Mingfeng Bai, PhD Andrew Berger, PhD  Arthur F. Gmitro, PhD  Martin Leahy, PhD
Brian Pogue, PhD Andries Zijlstra, PhD  Jason Valentine, PhD Ton van Leeuwen, PhD
Alex Walsh, PhD Christine Hendon, PhD  Nick Stone, PhD  Andrew Rollins, PhD
Wolfgang Drexler, PhD      
2017      
Jeffrey D. Schall, PhD William P. Roach, PhD Stephen J. Korn, PhD Doug Morgan, MD MPH
Jerry Wilmink, PhD Laura Waller, PhD Lauren Buchanan, PhD Antony Daniels, MD
Jeff Brooker Xiaoliang Sunny Xie, PhD Peter So, PhD Dr. Joseph Izatt, PhD
Dr. Cynthia Toth, MD Bruce Tromberg, PhD Justin Baba, PhD  
       
2016      
Matthew Lang, PhD Hilliel J. Chiel, PhD Eric Grogan, MD, MPH Dylan Burnette, PhD
Ji-Xin Cheng, PhD Matthew J. Baker, PhD Eric Betzig, PhD Caroline Boudoux, PhD 
Audrey Bowden, PhD Kristin Myers, PhD  Eric Skaar, PhD, MPH Brian J. Wong, MD, PhD, FACS 
Curtiss Marcott, PhD Jay Anderson, BS  Paul Stoddart, PhD  Joshua Caldwell, PhD 
Qi Zhang, PhD J. Micheal Newton, MD, PhD  Daniel Palanker, PhD   
       
2015      
Eric R. Tkacyk, MD, PhD Xingde Li, PhD Vasan Venugopalan, ScD Andrew Dunn, PhD
Madan Jagasia, MD Mark Canales, PhD Hillel J. Chiel, PhD  

  Sponsors

SPIE Princeton
   
Andor Renishaw
   
AcFee  

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