CSB Spotlight: Prashant Singh, Iverson Lab

Posted by daviskd2 on Friday, May 30, 2025 in News.

Prashant Singh, is a Senior Research Associate in the Iverson Lab. He’s getting used to the spotlight from his recent appearance on the YouTube channel SmarterEveryDay.

However, he’s still the down-to-earth Prash, as he’s known to his friends and family, who is always ready to respond to the “call of duty.”

Meet Prash …

What is the focus of your research?

My research focuses on understanding the structure and function of macromolecular assemblies, such as bacterial flagellar motors and membrane-bound mitochondrial proteins, using cryo-electron microscopy (cryoEM). In one of my current projects, we are collaborating with Caleb Hayes in Dr. Vivian Gama’s lab to investigate how mitochondrial proteins are organized to support the organelle’s architecture and function, using cryo-electron tomography (cryoET).

What tools/techniques do you use?

To understand protein structure and function, I use cryoEM, X-ray crystallography, surface plasmon resonance, mass spectrometry, and analytical ultracentrifugation.

What was your path to this position?

After earning my master’s degree from MTSU, I joined Dr. Iverson’s lab as a Research Assistant and trained in crystallography. I then moved to Dr. Melanie Ohi’s lab, where I served as laboratory manager and trained in negative-stain electron microscopy. I returned to Dr. Iverson’s lab to lead cryoEM projects, build infrastructure, and mentor researchers.

What is your favorite part of performing research within a lab?

The freedom to explore. I can wake up with an idea and begin testing it that very day, thanks to the tools, resources, and collaborative environment in the lab and at Vanderbilt.

What do you think is the key to a cohesive lab environment?

Curiosity, trust, and freedom. A lab thrives when its members are driven by curiosity, feel safe to ask questions, and are encouraged to pursue their own ideas.

What are some fun activities you like to do outside the lab?

I enjoy hiking with my family, cycling with friends, creating scientific animations, and—after submitting a paper—playing Call of Duty non-stop for a few days!