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pS219V

Allows expression of N-terminally His-tagged TEV protease in E. coli.
TEV protease recognition sequence: ENLYFQ↓G

  • Ref: Protein Eng. 14:993-1000 (2001)
  • tac promoter
  • low copy
  • Amp resistance
  • Encodes Ser219 → Val point mutant of the TEV protease catalytic domain
  • S219V mutant has been shown to be more stable and more efficient as a catalyst than the wild-type protease
  • pS219V produces an MBP-His-TEV fusion protein with an internal cleavage site
  • The product self-cleaves intracellularly to yield His-tagged protease, MW = 28.5 kDa