John A. McLean
Title and Contact Information
Stevenson Professor of Chemistry
Ph.D., The George Washington University, 2001
In the News
Journal- Molecular BioSystems
Research News @ Vanderbilt- New technique maps twin faces of smallest Janus nanoparticles
Arts and Science- John McLean was awarded Excellence in Teaching
Arts and Science- That Alcohol Is Going On Your Permanent Record
Arts and Science- And the Award Goes to
Arts and Science Fall 2008- And the Award Goes to
Research News @ Vanderbilt- Vanderbilt-led team to develop 'microbrain' to improve drug testing
Research News @ Vanderbilt- New tool for mining bacterial genome for novel drugs
Research News @ Vanderbilt- Vanderbilt awarded $16.5 million agreement to determine how toxic agents affect human cells
Research News @ Vanderbilt- 'Thought Leader' named for ion mobility mass spectrometry research
Bioanalytical and Biophysical Chemistry
Our research focuses on the design, construction, and application of advanced technologies for structural mass spectrometry, in particular, for studies in structural proteomics, systems biology, and biophysics. To identify and structurally characterize biomolecules from complex samples, we perform rapid (µs-ms) two-dimensional gas-phase separations using ion mobility-mass spectrometry (IM-MS) techniques. IM-MS provides separations on the basis of apparent surface area (ion-neutral collision cross section) and mass-to-charge (m/z), respectively. Biomolecular structural information is interpreted by comparing experimentally obtained collision cross-sections in the context of those obtained via molecular dynamics simulations.
Figure 1. (A) An illustration of the conformation space separation of different classes of biomolecules. Note that the indicated trends in the correlation function of collision cross section and m/z for particular molecular classes are for qualitative illustration purposes. Structural motifs (secondary structural elements, intramolecular solvation of post-translational modifications, etc.) give rise to deviation from the average correlation function within a particular molecular class.
In the analysis of complex biological materials, IM-MS provides a significant advantage over contemporary MS in that the regions in which signals appear in 2D conformation-space correspond with specific molecular class, i.e. the correlation of collision cross section with m/z varies as nucleotides/carbohydrates < peptides/ proteins < lipids/surfactants (Figure 1). Deviations from where a particular signal is predicted to occur can provide additional information including: (i) identification of sights of post-translational modification, (ii) characterization of secondary, tertiary, and quaternary structural motifs, and (iii) rapid screening for analyte-ligand binding interactions. The separation of analytes on the basis of molecular class provides significant advantages as a proteomics tool, because signals arising from concomitant, non-peptidic, materials are readily separated from the peptides of interest. For example, Figure 2 shows a representative IM-MS plot of conformation-space for an HPLC fraction of an E. Coli whole-cell lysate. Signals arising from surfactant contaminant in the sample preparation are resolved from peptides on the basis of structure and can thus be eliminated when searching peptide m/z signals against proteomic and genomic databases for high-confidence level protein identification.
Figure 2. A 3D plot of IM-MS conformation space for a representative E. Coli whole-cell lysate fraction measured by HPLC-MALDI-IM-TOFMS. Two distinct trends of correlated arrival time distribution (which is the observable measured and subsequently transformed to collision-cross section) vs. m/z are obtained corresponding to non-peptidic concomitant species and tryptic peptides, respectively.
Importantly, IM-MS can provide detailed structural information for conformational sub-populations of the same analyte (Figure 3), i.e. the relative abundances of different biomolecular conformations can be readily determined. By measuring the change in relative abundance of specific structural conformations as a function of IM separation temperature, thermodynamic and kinetic parameters can also be determined for structural sub-populations, or for structural transitions, respectively (i.e. via van't Hoff or Arrhenius plots). Note that this procedure can also be used to quantify the thermodynamic consequences of stepwise addition of solvent, or small molecules, on the prevailing molecular structure.
Figure 3. Experimental IM-MS conformation-space for a model peptide exhibiting two distinct structural sub-populations. Structures obtained by molecular dynamics simulations indicate helical and compact structures consistent with these results.
Key areas of investigation and development include:
- Investigation of the prevailing influences of post-translational modifications (e.g. glycosylation, phosphorylation, ubiquination, sumoylation, etc.) on protein secondary and tertiary structure?
- Development of imaging IM-MS instrumentation for multidimensional imaging/characterization of biomarker species from thin tissue sections and microarrays.
- Combining microfluidic separation strategies with IM-MS for fundamental biomolecular characterization in systems biology.
- Development of selective IM-MS shift reagents for high confidence level identification in proteomics, glycomics, lipidomics, and metabolomics.
Development of molecular dynamics strategies for structural interpretation of complementary IM-MS collision cross sections.
- Construction of advanced laser optical strategies for high-throughput screening of drug-ligand interactions.