John P. Wikswo, Jr.

Gordon A. Cain University Professor
A.B. Learned Professor of Living Physics
Professor of Physics, Biomedical Engineering, and Molecular Physiology and Biophyiscs
Director, Vanderbilt Institute for Integrative Biosystems Research and Education

Overview:

The Vanderbilt Institute for Integrative Biosystems Research and Education (VIIBRE) is developing nanophysiometers for measurements on single cells and nanobioreactors for perfusing small populations of cells in multiple cell traps and organs-on-chips. VIIBRE’s nanobioreactors are being interfaced to nanoelectrospray, ion mobility-mass spectrometers for real-time metabolomic analyses. The sensor and potentiostat technologies developed by the Cliffel and Wikswo collaboration are immediately applicable to the measurement of glucose, lactate, oxygen, and pH levels in cells in multitrap nanophysiometers and organs on chips, allowing the assessment of dynamic metabolic activity in response to specific challenges delivered in vitro. Optical sensing and imaging platforms developed by VIIBRE and its collaborators couple into the sub-nanoliter space for rapid customization of specific fluorescent and luminescent assays. The use of optical probes in combination with the nanophysiometer allows the integration of novel sensing mechanisms to monitor cell physiology and environmental toxicology. VIIBRE has contributed to the development of a system for time-resolved spectroscopic imaging of nanoparticles and nanoclusters, and for studying protein complexes in single cells.

Awards:

Fellow, American Association for the Advancement of Science (AAAS), 2010
Fellow, Institute for Electrical and Electronic Engineers, 2008
Fellow, Heart Rhythm Society, 2006
The Nightingale Prize, 2006
Fellow, Biomedical Engineering Society, 2005
Fellow of the American Heart Association, 2001
Fellow, American Institute for Medical and Biological Engineering, 1999
Thomas Jefferson Award, Vanderbilt University, 1997
John Simon Guggenheim Fellow, 1992-1993
Fellow, American Physical Society, 1990
IR-100 Award for Neuromagnetic Current Probe, 1984
Alfred P. Sloan Research Fellow, 1980-1982

Selected Publications:

The microfluidic multitrap nanophysiometer for hematologic cancer cell characterization reveals temporal sensitivity of the calcein-AM efflux assay. Byrd, TF; Hoang, LT; Kim, EG; Pfister, ME; Werner, EM; Arndt, SE; Chamberlain, JW; Hughey, JJ; Nguyen, BA; Schneibel, EJ; Wertz, LL; Whitfield, JS; Wikswo, JP; Seale, KT, SCIENTIFIC REPORTS, 4, 5117 , (2014) View Abstract

Single-Nanocrystal Spectroscopy of White-Light-Emitting CdSe Nanocrystals. Dukes, AD, Samson, PC, Keene, JD, Davis, LM, Wikswo, JP, Rosenthal, SD, JOURNAL OF PHYSICAL CHEMISTRY, 115, 4076-4081 , (2011) View Abstract

A microfabricated nanocalorimeter: Design, characterization, and chemical calibration. Xu, J; Reiserer, R; Tellinghuisen, J; Wikswo, JP; Baudenbacher, FJ, ANALYTICAL CHEMISTRY, 80, 2728-2733 , (2008) View Abstract

Quantum dot probes for monitoring dynamic cellular response: Reporters of T cell activation. Warnement, MR; Faley, SL; Wikswo, JP; Rosenthal, SJ, IEEE TRANSACTIONS ON NANOBIOSCIENCE, 5, 268-272 , (2006) View Abstract

NanoLiterBioReactor: Long-term mammalian cell culture at nanofabricated scale. Prokop, A; Prokop, Z; Schaffer, D; Kozlov, E; Wikswo, J; Cliffel, D; Baudenbacher, F, BIOMEDICAL MICRODEVICES, 6, 325-339 , (2004) View Abstract