{"id":2524,"date":"2024-03-26T13:15:07","date_gmt":"2024-03-26T13:15:07","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/evolution\/?p=2524"},"modified":"2024-09-10T14:35:55","modified_gmt":"2024-09-10T14:35:55","slug":"beckman-scholar-charu-balamurugan-unveils-evolutionary-insights-into-penicillium-secondary-metabolites","status":"publish","type":"post","link":"https:\/\/www.vanderbilt.edu\/evolution\/beckman-scholar-charu-balamurugan-unveils-evolutionary-insights-into-penicillium-secondary-metabolites\/","title":{"rendered":"Beckman Scholar Charu Balamurugan Unveils Evolutionary Insights into Penicillium Secondary Metabolites"},"content":{"rendered":"

By: Andy Flick, Evolutionary Studies Scientific Coordinator<\/em><\/p>\n

Beckman Scholar<\/a> Charu Balamurugan<\/a>, an undergraduate researcher in the Rokas lab<\/a> at Vanderbilt University, has shed light on the intricate evolutionary patterns governing secondary metabolite biosynthesis in fungi. Working with former Ph.D. student Jacob Steenwyk, lab PI Antonis Rokas<\/a>, and collaborator and Global Scholars Program participant Gustavo Goldman<\/a>, Balamurugan published the article The evolution of the gliotoxin biosynthetic gene cluster in <\/em>Penicillium fungi<\/em><\/a> in the journal “G3: Genes|Genomes|Genetics.” Balamurugan delved deep into the genomes of Penicillium<\/em> species, uncovering fascinating insights into the conservation and evolution of biosynthetic gene clusters (BGCs) and associated resistance genes.<\/p>\n

\"Charu'sFungi produce a diverse array of secondary metabolites, which play crucial roles in ecological interactions and human affairs. For example, Penicillium <\/em>molds are famous because they include the species in which Alexander Fleming discovered penicillin, the world\u2019s first antibiotic. Another secondary metabolite that has garnered significant attention due to its biomedical relevance is gliotoxin, a potent immunosuppressant. Gliotoxin is so toxic that fungal species producing it often contain genes for resistance to gliotoxin. Balamurugan’s study focused on understanding the evolutionary conservation of homologs of the gliotoxin genetic pathway and genes involved in gliotoxin resistance across Penicillium<\/em> species.<\/p>\n

According to Goldman, \u201cvery little is known about the distribution of the genes involved in gliotoxin biosynthesis and resistance in filamentous fungi.\u201d<\/p>\n

Analyzing 35 Penicillium<\/em> genomes from 23 species, Balamurugan and her colleagues found that some species harbored homologous, lesser fragmented gliotoxin BGCs, while others contained fragmented remnants or lacked the pathway altogether. Surprisingly, broad conservation of resistance genes was observed across Penicillium<\/em> genomes.<\/p>\n

\"Charu\u201cThe most interesting result of this work is that the genes encoded in BGCs\u2014like that of gliotoxin\u2014can have complex histories and be retained in genomes long after the loss of secondary metabolite biosynthesis, such as among Penicillium<\/em> species,\u201d she said. \u201cMore broadly, among families like Aspergillaceae, where there exists a mix of pathogenic and non-pathogenic species, beginning to trace the evolution of important factors of pathogenicity, like gliotoxin, are especially important.\u201d<\/p>\n

Balamurugan\u2019s mentor and co-author on this study, Steenwyk, continued, \u201csurprisingly, while the genes responsible for producing certain toxins in various Penicillium<\/em> species show a patchwork of presence and absence, the genes that confer resistance to certain potent compounds are broadly conserved across different species. This suggests that, even after losing the ability to produce certain secondary metabolites, fungi retain their defenses against them. This may be a signature of how fungi survive chemical warfare.\u201d<\/p>\n

Evolutionary rate analyses suggested that some of the remaining genes of the gliotoxin genetic pathway are functional or lost their function so recently that they have yet to accumulate inactivating mutations. Analyses of the evolutionary history of gliotoxin genes revealed a complex history of gene duplications, losses, and horizontal gene transfers, highlighting the dynamic nature of fungal secondary metabolite biosynthesis.<\/p>\n

Balamurugan’s journey of excellence at Vanderbilt University is marked by a trail of accomplishments and profound impact. Notably featured on VU’s Instagram Four with a ‘Dore<\/em><\/a> series, Balamurugan’s visionary leadership showed through her founding of Agni, Vanderbilt’s premier South Asian classical dance collective, demonstrating her commitment to cultural enrichment and artistic expression. Balamurugan’s dedication and aptitude earned her recognition as a Data Science Institute Summer Research Program Fellow, solidifying her prowess in scientific inquiry and innovation.<\/p>\n

In addition to her academic pursuits, Balamurugan is a beacon of social consciousness and activism. As an Ingram Scholar<\/a>, she champions equity and inclusivity, embodying Vanderbilt’s ethos of fostering a diverse and equitable community. Her involvement with Active Minds at Vanderbilt underscores her commitment to mental health advocacy, where she raises awareness and reduces stigma surrounding mental illness. In her Medicine, Health, and Society<\/em><\/a> program, she investigated the issue of mental health among incarcerated youth.<\/p>\n

Balamurugan epitomizes the core values of Vanderbilt University: academic excellence, social responsibility, and compassionate leadership. Through her multifaceted contributions and unwavering dedication, she continues to inspire and uplift the Vanderbilt community, leaving an indelible mark on campus and beyond.<\/p>\n

According to Steenwyk, \u201cCharu is an exceptionally talented undergraduate and working with her has been a remarkably enriching experience. Charu brings fresh perspectives and ambitious curiosity to her research. She is a testament to the vibrant future of science.\u201d<\/p>\n

Jeffrey Johnston, Beckman Scholars Program Director, continued, \u201cCharu is a rocketing student-scientist who gracefully navigates within an exceptionally talented crowd. She has blazed a trail as an outstanding student and a dedicated experimentalist that the VU Beckman Scholars Program recognized. Her latest publication continues her growing record of scientific impact in evolutionary studies.\u201d<\/p>\n

Citation: Balamurugan, C., Steenwyk, J.L., Goldman, G.H., Rokas, A. The evolution of the gliotoxin biosynthetic gene cluster in Penicillium<\/em> fungi. 2024. G3 Genes|Genomes|Genetics. https:\/\/doi.org\/10.1093\/g3journal\/jkae063<\/a><\/p>\n

Funding Statement: C.B. was supported as a Beckman Scholar by the Arnold and Mabel Beckman Foundation. J.L.S. is a Howard Hughes Medical Institute Awardee of the Life Sciences Research Foundation. Research in the Rokas lab is supported by grants from the National Science Foundation (DEB – 2110404), the National Institutes of Health\/National Institute of Allergy and Infectious Diseases (R01 AI153356), and the Burroughs Wellcome Fund.<\/p>\n","protected":false},"excerpt":{"rendered":"

By: Andy Flick, Evolutionary Studies Scientific Coordinator Beckman Scholar Charu Balamurugan, an undergraduate researcher in the Rokas lab at Vanderbilt University, has shed light on the intricate evolutionary patterns governing secondary metabolite biosynthesis in fungi. Working with former Ph.D. student Jacob Steenwyk, lab PI Antonis Rokas, and collaborator and Global Scholars Program participant Gustavo Goldman,…<\/p>\n","protected":false},"author":2421,"featured_media":2525,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"spay_email":"","jetpack_publicize_message":"","jetpack_is_tweetstorm":false,"jetpack_publicize_feature_enabled":true},"categories":[1],"tags":[13,14,104,16,15,105,88],"acf":[],"yoast_head":"\n\n\n\n\n\n\n\n\n\n\n\n\t\n\t\n\t\n\n\n\t\n\t\n\t\n