CSB Research: Tilting retromer to improve the model
Does your protein of interest show favored orientations in cryo-EM? Or perhaps one specific region is poorly resolved relative to another? If so, consider looking into this recent publication from Amy Kendall, Mintu Chandra, Will Wan and Lauren Jackson.
Their findings, published in the Journal of Biological Chemistry, report an improved model of the retromer trafficking protein by collecting data with a ±15° tilt. When combined with their previous data, these tilted data sets
increased resolution and revealed more complete interaction interfaces.
Biologically, trafficking proteins play a vital role in cells to help diverse cargo fold correctly and travel to their proper compartments. Dysregulation of these processes contributes to neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease.
Ultimately, these improved retromer models could lead to testable hypotheses relating to retromer function and its role in disease. Read the article here! ~S.D.W.