{"id":585,"date":"2018-07-09T13:10:26","date_gmt":"2018-07-09T19:10:26","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/cit\/?page_id=585"},"modified":"2024-01-25T14:13:57","modified_gmt":"2024-01-25T20:13:57","slug":"full-service-shared-resource-core","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/cit\/full-service-shared-resource-core\/","title":{"rendered":"Metabolomics Study Design &#8211; Workflows"},"content":{"rendered":"<div  style='padding-bottom:10px; ' class='av-special-heading av-special-heading-h3    avia-builder-el-0  el_before_av_textblock  avia-builder-el-first  '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >4 &#8211; General Workflows at the CIT<\/h3><div class='special-heading-border'><div class='special-heading-inner-border' ><\/div><\/div><\/div>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p>As a full-service shared resource core we consider our clients as collaborators and provide many custom applications. <strong>Figure 1<\/strong>\u00a0provides an overview of the general small molecule omic workflows for hypothesis-generating (global untargeted) and hypothesis-validating (targeted) assays.<\/p>\n<\/div><\/section>\n<div  style='height:20px' class='hr hr-invisible   avia-builder-el-2  el_after_av_textblock  el_before_av_one_half '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div>\n<div class=\"flex_column av_one_half  no_margin flex_column_div first  avia-builder-el-3  el_after_av_hr  el_before_av_one_half  \" style='padding:0px 20px 0px 0px ; border-radius:0px; '><p><div  class='av-hotspot-image-container   av-hotspot-numbered av-mobile-fallback-active  av-permanent-tooltip  avia-builder-el-4  el_before_av_hr  avia-builder-el-first  av-non-fullwidth-hotspot-image'  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class='av-hotspot-container'><div class='av-hotspot-container-inner-cell'><div class='av-hotspot-container-inner-wrap'><div class='av-image-hotspot av-image-hotspot-1 av-permanent-tooltip' data-avia-tooltip-position='top' data-avia-tooltip-alignment='left' data-avia-tooltip-class='av-tt-default-width av-tt-pos-above av-tt-align-left  av-mobile-fallback-active  av-permanent-tooltip-single  main_color av-tt-hotspot' data-avia-tooltip='&lt;p&gt;&lt;a href=&quot;https:\/\/www.vanderbilt.edu\/cit\/global-untargeted-targeted-metabolomics\/&quot;&gt;&lt;strong&gt;Untargeted or Targeted&lt;\/strong&gt;&lt;\/a&gt;&lt;\/p&gt;\n' style='top: 46.9%; left: 52.4%; '><a href=https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-replicates\/  class='av-image-hotspot_inner' style=' '>1<\/a><div class='av-image-hotspot-pulse' ><\/div><\/div><div class='av-image-hotspot av-image-hotspot-2 av-permanent-tooltip' data-avia-tooltip-position='bottom' data-avia-tooltip-alignment='right' data-avia-tooltip-class='av-tt-default-width av-tt-pos-below av-tt-align-right  av-mobile-fallback-active  av-permanent-tooltip-single  main_color av-tt-hotspot' data-avia-tooltip='&lt;p&gt;&lt;a href=&quot;https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-replicates\/&quot;&gt;&lt;strong&gt;Replicates &amp; Volumes&lt;\/strong&gt;&lt;\/a&gt;&lt;\/p&gt;\n' style='top: 62.2%; left: 53.9%; '><a href=https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-storage-and-handling\/  class='av-image-hotspot_inner' style=' '>2<\/a><div class='av-image-hotspot-pulse' ><\/div><\/div><div class='av-image-hotspot av-image-hotspot-3 av-permanent-tooltip' data-avia-tooltip-position='top' data-avia-tooltip-alignment='left' data-avia-tooltip-class='av-tt-default-width av-tt-pos-above av-tt-align-left  av-mobile-fallback-active  av-permanent-tooltip-single  main_color av-tt-hotspot' data-avia-tooltip='&lt;p&gt;&lt;a href=&quot;https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-storage-and-handling\/&quot;&gt;&lt;strong&gt;Handling &amp; Storage&lt;\/strong&gt;&lt;\/a&gt;&lt;\/p&gt;\n' style='top: 60%; left: 72.3%; '><a href=https:\/\/www.vanderbilt.edu\/cit\/global-untargeted-targeted-metabolomics\/  class='av-image-hotspot_inner' style=' '>3<\/a><div class='av-image-hotspot-pulse' ><\/div><\/div><div class='av-image-hotspot av-image-hotspot-4 av-permanent-tooltip' data-avia-tooltip-position='bottom' data-avia-tooltip-alignment='left' data-avia-tooltip-class='av-tt-default-width av-tt-pos-below av-tt-align-left  av-mobile-fallback-active  av-permanent-tooltip-single  main_color av-tt-hotspot' data-avia-tooltip='&lt;p&gt;&lt;a href=&quot;https:\/\/www.vanderbilt.edu\/cit\/full-service-shared-resource-core\/&quot;&gt;&lt;b&gt;Workflows&lt;\/b&gt;&lt;\/a&gt;&lt;\/p&gt;\n' style='top: 52.3%; left: 10.2%; '><a href=https:\/\/www.vanderbilt.edu\/cit\/full-service-shared-resource-core\/  class='av-image-hotspot_inner' style=' '>4<\/a><div class='av-image-hotspot-pulse' ><\/div><\/div><div class='av-image-hotspot av-image-hotspot-5 av-permanent-tooltip' data-avia-tooltip-position='top' data-avia-tooltip-alignment='left' data-avia-tooltip-class='av-tt-default-width av-tt-pos-above av-tt-align-left  av-mobile-fallback-active  av-permanent-tooltip-single  main_color av-tt-hotspot' data-avia-tooltip='&lt;p&gt;&lt;a href=&quot;https:\/\/www.vanderbilt.edu\/cit\/metabolite-identification-confidence-levels\/&quot;&gt;&lt;strong&gt;ID Confidence Levels&lt;\/strong&gt;&lt;\/a&gt;&lt;\/p&gt;\n' style='top: 15.5%; left: 21.8%; '><a href=https:\/\/www.vanderbilt.edu\/cit\/metabolite-identification-confidence-levels\/  class='av-image-hotspot_inner' style=' '>5<\/a><div class='av-image-hotspot-pulse' ><\/div><\/div><img class='wp-image-1583 avia-img-lazy-loading-not-1583 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140908\/IMG_6580c_cutout_small_lowerres-1030x654-1.jpg\" alt='' title='IMG_6580c_cutout_small_lowerres-1030x654'  height=\"654\" width=\"1030\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140908\/IMG_6580c_cutout_small_lowerres-1030x654-1.jpg 1030w, https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140908\/IMG_6580c_cutout_small_lowerres-1030x654-1-300x190.jpg 300w, https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140908\/IMG_6580c_cutout_small_lowerres-1030x654-1-768x488.jpg 768w, https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140908\/IMG_6580c_cutout_small_lowerres-1030x654-1-705x448.jpg 705w\" sizes=\"(max-width: 1030px) 100vw, 1030px\" \/><\/div><\/div><\/div><div class='av-hotspot-fallback-tooltip av-image-hotspot-1 av-permanent-tooltip'><div class='av-hotspot-fallback-tooltip-count'>1<div class='avia-arrow'><\/div><\/div><div class='av-hotspot-fallback-tooltip-inner clearfix'><p>\n<a href=\"https:\/\/www.vanderbilt.edu\/cit\/global-untargeted-targeted-metabolomics\/\"><strong>Untargeted or Targeted<\/strong><\/a><\/p>\n<\/div><\/div><div class='av-hotspot-fallback-tooltip av-image-hotspot-2 av-permanent-tooltip'><div class='av-hotspot-fallback-tooltip-count'>2<div class='avia-arrow'><\/div><\/div><div class='av-hotspot-fallback-tooltip-inner clearfix'><p>\n<a href=\"https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-replicates\/\"><strong>Replicates &amp; Volumes<\/strong><\/a><\/p>\n<\/div><\/div><div class='av-hotspot-fallback-tooltip av-image-hotspot-3 av-permanent-tooltip'><div class='av-hotspot-fallback-tooltip-count'>3<div class='avia-arrow'><\/div><\/div><div class='av-hotspot-fallback-tooltip-inner clearfix'><p>\n<a href=\"https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-storage-and-handling\/\"><strong>Handling &amp; Storage<\/strong><\/a><\/p>\n<\/div><\/div><div class='av-hotspot-fallback-tooltip av-image-hotspot-4 av-permanent-tooltip'><div class='av-hotspot-fallback-tooltip-count'>4<div class='avia-arrow'><\/div><\/div><div class='av-hotspot-fallback-tooltip-inner clearfix'><p>\n<a href=\"https:\/\/www.vanderbilt.edu\/cit\/full-service-shared-resource-core\/\"><b>Workflows<\/b><\/a><\/p>\n<\/div><\/div><div class='av-hotspot-fallback-tooltip av-image-hotspot-5 av-permanent-tooltip'><div class='av-hotspot-fallback-tooltip-count'>5<div class='avia-arrow'><\/div><\/div><div class='av-hotspot-fallback-tooltip-inner clearfix'><p>\n<a href=\"https:\/\/www.vanderbilt.edu\/cit\/metabolite-identification-confidence-levels\/\"><strong>ID Confidence Levels<\/strong><\/a><\/p>\n<\/div><\/div><\/div><br \/>\n<div  style='height:20px' class='hr hr-invisible   avia-builder-el-5  el_after_av_image_hotspot  el_before_av_textblock  avia-builder-el-first '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><br \/>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p>Once samples are received, CIT personnel will proceed with 1) sample preparation, 2) data acquisition, 3) data processing and analysis, and 4) report generation.\u00a0 The CIT staff aims to tailor data output(s), reports and spreadsheets based on client discussions. We perform <a href=\"https:\/\/www.vanderbilt.edu\/cit\/omics-data-analysis\/\">numerous basic and advanced data processing tasks<\/a>.<\/p>\n<\/div><\/section><\/p><\/div><div class=\"flex_column av_one_half  no_margin flex_column_div av-zero-column-padding   avia-builder-el-7  el_after_av_one_half  el_before_av_hr  \" style='border-radius:0px; '><div  class='avia-image-container avia_animated_image avia_animate_when_almost_visible pop-up av-styling- av-hover-grow   avia-builder-el-8  avia-builder-el-no-sibling  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='wp-image-1582 avia-img-lazy-loading-not-1582 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140802\/CIT-Molecular-Omics-Workflows.jpg\" alt='' title='CIT Molecular Omics Workflows' height=\"648\" width=\"700\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140802\/CIT-Molecular-Omics-Workflows.jpg 700w, https:\/\/cdn.vanderbilt.edu\/vu-URL\/wp-content\/uploads\/sites\/166\/2024\/01\/25140802\/CIT-Molecular-Omics-Workflows-300x278.jpg 300w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/div><\/div><\/div><\/div><div  style='height:60px' class='hr hr-invisible   avia-builder-el-9  el_after_av_one_half  el_before_av_textblock '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><\/p>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><h5>Read our other tips to get started with metabolomics analyses:<\/h5>\n<\/div><\/section>\n<div class=\"flex_column av_one_fourth  flex_column_div av-zero-column-padding first  avia-builder-el-11  el_after_av_textblock  el_before_av_one_fourth  column-top-margin\" style='border-radius:0px; '><\/div>\n<div class=\"flex_column av_one_fourth  flex_column_div av-zero-column-padding   avia-builder-el-12  el_after_av_one_fourth  el_before_av_one_fourth  column-top-margin\" style='border-radius:0px; '><p><div  class='avia-button-wrap avia-button-center  avia-builder-el-13  el_before_av_hr  avia-builder-el-first ' ><a href='https:\/\/www.vanderbilt.edu\/cit\/global-untargeted-targeted-metabolomics\/' class='avia-button avia-button-fullwidth   avia-icon_select-no avia-color-theme-color '  style='color:#ffffff; ' ><span class='avia_iconbox_title' >1. Discovery or Validation?<\/span><span class='avia_button_background avia-button avia-button-fullwidth avia-color-blue' ><\/span><\/a><\/div><br \/>\n<div  style='height:20px' class='hr hr-invisible   avia-builder-el-14  el_after_av_button_big  el_before_av_button_big  avia-builder-el-no-sibling '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><br \/>\n<div  class='avia-button-wrap avia-button-center  avia-builder-el-15  el_after_av_hr  avia-builder-el-last ' ><a href='https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-replicates\/' class='avia-button avia-button-fullwidth   avia-icon_select-no avia-color-theme-color '  style='color:#ffffff; ' ><span class='avia_iconbox_title' >2. Sample Replicates and Volumes<\/span><span class='avia_button_background avia-button avia-button-fullwidth avia-color-blue' ><\/span><\/a><\/div><\/p><\/div>\n<div class=\"flex_column av_one_fourth  flex_column_div av-zero-column-padding   avia-builder-el-16  el_after_av_one_fourth  el_before_av_one_fourth  column-top-margin\" style='border-radius:0px; '><p><div  class='avia-button-wrap avia-button-center  avia-builder-el-17  el_before_av_hr  avia-builder-el-first ' ><a href='https:\/\/www.vanderbilt.edu\/cit\/metabolomics-sample-storage-and-handling\/' class='avia-button avia-button-fullwidth   avia-icon_select-no avia-color-theme-color '  style='color:#ffffff; ' ><span class='avia_iconbox_title' >3. Sample Handling and Storage<\/span><span class='avia_button_background avia-button avia-button-fullwidth avia-color-blue' ><\/span><\/a><\/div><br \/>\n<div  style='height:20px' class='hr hr-invisible   avia-builder-el-18  el_after_av_button_big  el_before_av_button_big  avia-builder-el-no-sibling '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><br \/>\n<div  class='avia-button-wrap avia-button-center  avia-builder-el-19  el_after_av_hr  avia-builder-el-last ' ><a href='https:\/\/www.vanderbilt.edu\/cit\/metabolite-identification-confidence-levels\/' class='avia-button avia-button-fullwidth   avia-icon_select-no avia-color-theme-color '  style='color:#ffffff; ' ><span class='avia_iconbox_title' >5. Identification Confidence Levels<\/span><span class='avia_button_background avia-button avia-button-fullwidth avia-color-blue' ><\/span><\/a><\/div><\/p><\/div>\n<div class=\"flex_column av_one_fourth  flex_column_div av-zero-column-padding   avia-builder-el-20  el_after_av_one_fourth  el_before_av_hr  column-top-margin\" style='border-radius:0px; '><\/div>\n<div  style='height:60px' class='hr hr-invisible   avia-builder-el-21  el_after_av_one_fourth  el_before_av_social_share '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div>\n<div  class='av-social-sharing-box  avia-builder-el-22  el_after_av_hr  avia-builder-el-last  '><div class='av-share-box'><h5 class='av-share-link-description av-no-toc '>Share this entry<\/h5><ul class='av-share-box-list noLightbox'><li class='av-share-link av-social-link-facebook' ><a target=\"_blank\" aria-label=\"Share on Facebook\" 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