{"id":583,"date":"2018-07-09T13:12:12","date_gmt":"2018-07-09T19:12:12","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/cit\/?page_id=583"},"modified":"2024-01-25T14:28:55","modified_gmt":"2024-01-25T20:28:55","slug":"global-untargeted-targeted-metabolomics","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/cit\/global-untargeted-targeted-metabolomics\/","title":{"rendered":"Metabolomics Study Design – Global Untargeted or Targeted Metabolomics?"},"content":{"rendered":"

1 – Untargeted or Targeted Metabolomics – Which Approach is Right for You?<\/h3>
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Metabolomics analyses can be divided into two main categories: 1) global untargeted<\/strong> and 2) targeted metabolomics<\/strong>. Each approach has its own advantages, applications and limitations as outlined below.<\/p>\n<\/div><\/section>\n

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\nUntargeted or Targeted<\/a><\/p>\n<\/div><\/div>

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\nReplicates & Volumes<\/a><\/strong><\/p>\n<\/div><\/div>

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\nHandling & Storage<\/strong><\/a><\/p>\n<\/div><\/div>

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\nWorkflows<\/strong><\/a><\/p>\n<\/div><\/div>

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\nID Confidence Levels<\/strong><\/a><\/p>\n<\/div><\/div><\/div>
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Targeted metabolomics<\/strong> enables validation and absolute quantification of a chosen subset of metabolites (e.g., metabolites within a specific pathway). As such, this method can also be used to complement an untargeted approach.<\/p>\n<\/div><\/section><\/p><\/div>

Global, untargeted metabolomics<\/strong> is hypothesis-generating. It is typically performed to collect and analyze all<\/em> detectable metabolites in a sample. As a discovery approach, global, untargeted analysis can also reveal novel compounds and chemical unknowns potentially leading to new insights in disease mechanism or treatment. As a result, various levels of detail<\/a><\/strong> and validation can be achieved.<\/p>\n<\/div><\/section>
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Molecular Omics Applications<\/div><\/div><\/div><\/p><\/div><\/p>\n
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Read our other tips to get started with metabolomics analyses:<\/h5>\n<\/div><\/section><\/div>\n
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2. Sample Replicates and Volumes<\/span><\/span><\/a><\/div>
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3. Sample Handling and Storage<\/span><\/span><\/a><\/div><\/p><\/div>\n

4. General Workflows at the CIT<\/span><\/span><\/a><\/div>
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5. Identification Confidence Levels<\/span><\/span><\/a><\/div><\/p><\/div>\n
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