The CIT has recently added an Agilent 6470 Triple Quadrupole (QqQ) LC/MS system to its lineup of state-of-the-art instrumentation. The components of this tandem system include two mass filters and a collision cell. Our Agilent 6470 Triple Quadrupole includes enhanced ion optics and detector design over other systems allowing for attogram level sensitivity.
This highly sensitive instrument is used for targeted analysis experiments, mainly multiple reaction monitoring (MRM), dynamic MRM (dMRM), and triggered MRM (tMRM). Through its increased sensitivity, wider linear range, and better signal-to-noise ratios it allows for lower detection limits. Most notably, the Triple Quadrupole allows for both relative and absolute quantification studies of metabolites, drugs, lipids, peptides, amino acids, and canonical metabolic pathways of interest. Example applications include biomarker validation, pharmaceuticals, steroids, toxicology/environmental analyses, and forensics.
However, please also note that generally, global untargeted analyses precede this type of analysis unless specific metabolic pathways or metabolites (i.e., TCA cycle, carnitine biosynthesis, amino acids, redox/energy molecules) are of interest to the user.
For a comparison of the various metabolomics instrumentation available at the CIT, please visit our Metabolomics page.