Liquid chromatography-mass spectrometry (LC-MS) tandem instruments offer the ability to separate compounds by both polarity (LC) and mass-to-charge (MS) dimensions. Consequently, this approach offers very high mass resolution and allows you to analyze compounds such as drug metabolites, biotechnological samples, and environmental toxins – even in complex matrices. A range of chromatography methods including reverse phase liquid chromatography (RP-LC) and hydrophilic interaction liquid chromatography (HILIC) can be used to optimize stationary phase retention.
The CIT houses a state-of-the-art LC-MS instrument, the Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). This instrument combines a segmented quadrupole for high-performance precursor ion selection with a high-resolution accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer offering a combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Our QE-HF is equipped with an ultra-high performance liquid chromatography system (Vanquish, ThermoFisher Scientific), nano- and conventional electrospray ionization (ESI) sources.
For a comparison of the various metabolomics instrumentation available at the CIT, please visit our Metabolomics page.