• Agilent LC/MS at Vanderbilt University
  • LC IM-MS
  • Waters Synapt G2-S at Vanderbilt University

The CIT houses several commercially available liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) instruments. These instruments offer the ability to separate ions or metabolites in three dimensions: polarity (LC), molecular size/shape/charge (IM), and mass-to-charge (MS). Consequently, they are ideal for low molecular weight analytes.

Our two traveling wave ion mobility-mass spectrometry (IM-MS) instruments (Synapt G2 HDMS and Synapt G2-S HDMS models, Waters Corporation) are coupled to Acquity UPLC or nanoAcquity UPLC systems. The G2-S HDMS model incorporates StepWave ion transfer optics and TriWave IM technologies for increased sensitivity. Both, Synapt nano- and conventional electrospray ionization (ESI) utilize a lockspray, which allows for mass calibration during data acquisition. In addition, a quadrupole using a collision-induced dissociation (CID) cell both directly before and after the traveling wave mobility cell can perform mass selection and fragmentation.

Our collaborative shared resource center also houses two state-of-the-art Agilent ion mobility instruments (Model 6560). These instruments were built with enhanced drift tube ion mobility resolution and precision. The 6560s are coupled to either a binary LC system (1260 Infinity, Agilent) or a microfluidic nano flow LC (i.e., Chip Cube) that utilizes columns on prepackaged chips for optimal reproducibility. The dual jet stream source is an electrospray ionization (ESI) source with increased sensitivity capabilities. For more specialized analyses, the static field ion mobility cell is capable of running in numerous gas conditions (i.e., nitrogen, helium and carbon dioxide) depending on the user’s needs.

For a comparison of the various metabolomics instrumentation available at the CIT, please visit our Metabolomics page.

Biomolecular Class Separation

Biomolecular Class Separation in MS

Higher Resolution Sub-Trends

Metabolite Collision Cross Section and Mass Data

Three Dimensions of Separation

Mass Spectrometry Metabolite Separation