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Cliffel, David E.
Associate Professor of Chemistry
Director, VINSE Biomolecular Nanostructures Facility
Member, VINSE Advisory Committee

Research Information

Multianalyte Microphysiometry. Analytical chemistry often leads the forefront of scientific discovery, as new instrumentation allows us to perform new experiments. Our group has created multianalyte microphysiometry by combining microfluidic technologies with electrochemical detection to study the dynamic behaviors of metabolic pathways. We are developing new multianalyte microphysiometry methods with applications in cancer, diabetes, and toxicology. We have added electrochemical detectors for many metabolic analytes into the microfluidic chamber to give a complete dynamical picture of the live cell population.

Nanoparticle Biomimics. The ability to mimic biomolecular recognition requires the generation of nanoscale structures that faithfully reproduce the lock & key motif of protein binding. We have designed biomimics that present targeted linear and loop peptide structures on the surface of monolayer protected gold nanoparticles. These biomimics are useful for calibrating immunoassays, especially in our immunosensor work with the quartz crystal microbalance.

Electrochemistry on the Nanoscale. Electron transfer in nanometer-size chemical systems is an important process for the creation of macromolecular electronics. We are interested in the electrochemistry of metallic nanoparticles, redox proteins like Photosystem I, and other electroactive nanomaterials that bridge the gap between bulk solids and discrete molecules in the development of electronic devices using nanotechnology.

Selected Publications

Modeling the measurements of cellular fluxes in microbioreactor devices using thin enzyme electrodes. Velkovsky, M; Snider, R; Cliffel, DE; Wikswo, JP, JOURNAL OF MATHEMATICAL CHEMISTRY, 49, 251-275 , (2011)

Multifunctional nanoparticles as simulants for a gravimetric immunoassay. Miller, SA; Hiatt, LA; Keil, RG; Wright, DW; Cliffel, DE, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 399, 1021-1029 , (2011)

A Structural Mass Spectrometry Strategy for the Relative Quantitation of Ligands on Mixed Monolayer-Protected Gold Nanoparticles. Harkness, KM; Hixson, BC; Fenn, LS; Turner, BN; Rape, AC; Simpson, CA; Huffman, BJ; Okoli, TC; McLean, JA; Cliffel, DE, ANALYTICAL CHEMISTRY, 82, 9268-9274 , (2010)

Characterization of thiolate-protected gold nanoparticles by mass spectrometry. Harkness, KM; Cliffel, DE; McLean, JA, ANALYST, 135, 868-874 , (2010)

Enhanced Photocurrent Production by Photosystem I Multilayer Assemblies. Ciesielski, PN; Faulkner, CJ; Irwin, MT; Gregory, JM; Tolk, NH; Cliffel, DE; Jennings, GK, ADVANCED FUNCTIONAL MATERIALS, 20, 4048-4054 , (2010)

Neuron specific metabolic adaptations following multi-day exposures to oxygen glucose deprivation. Zeiger, SLH; McKenzie, JR; Stankowski, JN; Martin, JA; Cliffel, DE; McLaughlin, B, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1802, 1095-1104 , (2010)

Photosystem I - Based biohybrid photoelectrochemical cells. Ciesielski, PN; Hijazi, FM; Scott, AM; Faulkner, CJ; Beard, L; Emmett, K; Rosenthal, SJ; Cliffel, D; Jennings, GK, BIORESOURCE TECHNOLOGY, 101, 3047-3053 , (2010)

Surface Fragmentation of Complexes from Thiolate Protected Gold Nanoparticles by Ion Mobility-Mass Spectrometry. Harkness, KM; Fenn, LS; Cliffel, DE; McLean, JA, ANALYTICAL CHEMISTRY, 82, 3061-3066 , (2010)

The Effects of Cholera Toxin on Cellular Energy Metabolism. Snider, RM; McKenzie, JR; Kraft, L; Kozlov, E; Wikswo, JP; Cliffel, DE, TOXINS, 2, 632-634 , (2010)

Tiopronin Gold Nanoparticle Precursor Forms Aurophilic Ring Tetramer. Simpson, CA; Farrow, CL; Tian, P; Billinge, SJL; Huffman, BJ; Harkness, KM; Cliffel, DE, INORGANIC CHEMISTRY, 49, 10858-10866 , (2010)

Unexpected Toxicity of Mono layer Protected Gold Clusters Eliminated by PEG-Thiol Place Exchange Reactions. Simpson, CA; Huffman, BJ; Gerdon, AE; Cliffel, DE, CHEMICAL RESEARCH IN TOXICOLOGY, 23, 1608-1616 , (2010)

Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer. Eklund, SE; Thompson, RG; Snider, RM; Carney, CK; Wright, DW; Wikswo, J; Cliffel, DE, SENSORS, 9, 2117-2133 , (2009)

The Role of Transforming Growth Factor-beta-Mediated Tumor-Stroma Interactions in Prostate Cancer Progression: An Integrative Approach. Basanta, D; Strand, DW; Lukner, RB; Franco, OE; Cliffel, DE; Ayala, GE; Hayward, SW; Anderson, ARA, CANCER RESEARCH, 69, 7111-7120 , (2009)

A multiwalled carbon nanotube/dihydropyran composite film electrode for insulin detection in a microphysiometer chamber. Snider, RM; Ciobanu, M; Rue, AE; Cliffel, DE, ANALYTICA CHIMICA ACTA, 609, 44-52 , (2008)

Design and synthesis of an antigenic mimic of the Ebola glycoprotein. Rutledge, RD; Huffman, BJ; Cliffel, DE; Wright, DW, JOURNAL OF MATERIALS RESEARCH, 23, 3161-3168 , (2008)

Diamond-derived ultramicroelectrodes designed for electrochemical analysis and bioanalyte sensing. Soh, KL; Kang, WP; Davidson, JL; Wong, YM; Cliffel, DE; Swain, GM, DIAMOND AND RELATED MATERIALS, 17, 900-905 , (2008)

Electrochemical impedance spectroscopy of synthetic pyrite doped with As, Co, and Ni. Lehner, S; Ciobanu, M; Savage, K; Cliffel, DE, JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 155, P61-P70 , (2008)

Functionalized Nanoporous Gold Leaf Electrode Films for the Immobilization of Photosystem I. Ciesielski, PN; Scott, AM; Faulkner, CJ; Berron, BJ; Cliffel, DE; Jennings, GK, ACS NANO, 2, 2465-2472 , (2008)

Glucose and lactate biosensors for scanning electrochemical microscopy imaging of single live cells. Ciobanu, M; Taylor, DE; Wilburn, JP; Cliffel, DE, ANALYTICAL CHEMISTRY, 80, 2717-2727 , (2008)

Ordered array of diamond ultramicroband electrodes for electrochemical analysis. Soh, KL; Kang, WP; Davidson, JL; Wong, YM; Cliffel, DE; Swain, GM, DIAMOND AND RELATED MATERIALS, 17, 240-246 , (2008)

Rapid assembly of photosystem I monolayers on gold electrodes. Faulkner, CJ; Lees, S; Ciesielski, PN; Cliffel, DE; Jennings, GK, LANGMUIR, 24, 8409-8412 , (2008)

Chemical and electrochemical oxidation of C8-arylamine adducts of 2 '-deoxyguanosine. Stover, JS; Ciobanu, M; Cliffel, DE; Rizzo, CJ, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 129, 2074-2081 , (2007)

Electrochemistry and photoelectrochemistry of photosystem I adsorbed on hydroxyl-terminated monolayers. Ciobanu, M; Kincaid, HA; Lo, V; Dukes, AD; Jennings, GK; Cliffel, DE, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 599, 72-78 , (2007)

Electrospray mass spectrometry study of tiopronin monolayer-protected gold nanoclusters. Gies, AP; Hercules, DM; Gerdon, AE; Cliffel, DE, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 129, 1095-1104 , (2007)

Mercury-free analysis of lead in drinking water by anodic stripping square wave voltammetry. Wilburn, JP; Brown, KL; Cliffel, DE, JOURNAL OF CHEMICAL EDUCATION, 84, 312-314 , (2007)

Output analysis of materials inkjet printer. Deravi, LF; Gerdon, AE; Cliffel, DE; Wright, DW; Sumerel, JL, APPLIED PHYSICS LETTERS, 91, 113114 , (2007)

The effect of As, Co, and Ni impurities on pyrite oxidation kinetics: An electrochemical study of synthetic pyrite. Lehner, S; Savage, K; Ciobanu, M; Cliffel, DE, GEOCHIMICA ET COSMOCHIMICA ACTA, 71, 2491-2509 , (2007)

A novel "coral" carbon microprobe with and without N2 incorporation

. Raina, S; Kang, WP; Davidson, JL; Bonds, AB; Cliffel, DE; Howell, MA, DIAMOND AND RELATED MATERIALS, 15, 1844-1848 , (2006)

Cell-based detection of biological toxins using metabolic microphysiometry. Cliffel, DE; Eklund, S; Snider, R, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 231, , (2006)

Cellular metabolic responses to anthrax protective antigen and lethal factor monitored with a multianalyte microphysiometer. Chambers, JR; Cliffel, DE, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 231, , (2006)

Detection of Ebola glycoprotein using quartz crystal microbalance immunosensor. Gerdon, AE; Huffman, BJ; Wright, DW; Cliffel, DE, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 231, , (2006)

Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. Yu, JS; Liao, HX; Gerdon, AE; Huffman, B; Scearce, RM; McAdams, M; Alam, SM; Popernack, PM; Sullivan, NJ; Wright, D; Cliffel, DE; Nabel, GJ; Haynes, BF, JOURNAL OF VIROLOGICAL METHODS, 137, 219-228 , (2006)

Electrochemical sensing using nanodiamond microprobe. Basu, S; Kang, WP; Davidson, JL; Choi, BK; Bonds, AB; Cliffel, DE, DIAMOND AND RELATED MATERIALS, 15, 269-274 , (2006)

Engineering challenges of BioNEMS: the integration of microfluidics, micro- and nanodevices, models and external control for systems biology. Wikswo, JP; Prokop, A; Baudenbacher, F; Cliffel, D; Csukas, B; Velkovsky, M, IEE PROCEEDINGS-NANOBIOTECHNOLOGY, 153, 81-101 , (2006)

Entrapment of photosystem I within self-assembled films. Kincaid, HA; Niedringhaus, T; Ciobanu, M; Cliffel, DE; Jennings, GK, LANGMUIR, 22, 8114-8120 , (2006)

Epitope mapping of the protective antigen of B-anthracis by using nanoclusters presenting conformational peptide epitopes. Gerdon, AE; Wright, DW; Cliffel, DE, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 45, 594-598 , (2006)

Imaging of voltage-gated alamethicin pores in a reconstituted bilayer lipid membrane via scanning electrochemical microscopy. Wilburn, JP; Wright, DW; Cliffel, DE, ANALYST, 131, 311-316 , (2006)

Multianalyte microphysiometry as a tool in metabolomics and systems biology. Eklund, SE; Snider, RM; Wikswo, J; Baudenbacher, F; Prokop, A; Cliffel, DE, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 587, 333-339 , (2006)

Scanning electrochemical microscopy determination of organic soluble MPC electron-transfer rates. Peterson, RR; Cliffel, DE, LANGMUIR, 22, 10307-10314 , (2006)

Continuous free-flow electrophoresis of water-soluble monolayer-protected clusters. Peterson, RR; Cliffel, DE, ANALYTICAL CHEMISTRY, 77, 4348-4353 , (2005)

Extracting metabolic fluxes from measurements with a multianalyte Microphysiometer. Velkovsky, M; Cliffel, D; Eklund, S; Eluvathingal, S; Stremler, M; Wikswo, J, BIOPHYSICAL JOURNAL, 88, 521A-522A , (2005)

Hemagglutinin linear epitope presentation on monolayer-protected clusters elicits strong antibody binding. Gerdon, AE; Wright, DW; Cliffel, DE, BIOMACROMOLECULES, 6, 3419-3424 , (2005)

Photosystem I patterning imaged by scanning electrochemical microscopy. Ciobanu, M; Kincaid, HA; Jennings, GK; Cliffel, DE, LANGMUIR, 21, 692-698 , (2005)

PT and Pd MPCs for use in asymmetric hydrogenation.. Moorhouse, R; Cliffel, DE; Ecklund, S, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 229, U520-U520 , (2005)

Quartz crystal microbalance detection of glutathione-protected nanoclusters using antibody recognition. Gerdon, AE; Wright, DW; Cliffel, DE, ANALYTICAL CHEMISTRY, 77, 304-310 , (2005)

Real-time cell dynamics with a multianalyte physiometer. Eklund, SE; Kozlov, E; Taylor, DE; Baudenbacher, R; Cliffel, DE, METHODS IN MOLECULAR BIOLOGY, 303, 209-223 , (2005)

A microphysiometer for simultaneous measurement of changes in extracellular glucose, lactate, oxygen, and acidification rate. Eklund, SE; Taylor, D; Kozlov, E; Prokop, A; Cliffel, DE, ANALYTICAL CHEMISTRY, 76, 519-527 , (2004)

Confirmed binding of single chain (SCFV) recombinant antibodies to zero valent metals with quartz crystal microbalance.. Gerdon, AE; Wright, DW; Cliffel, DE; Mernaugh, R, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 227, U1559-U1559 , (2004)

Diamond-derived microelectrodes array for electrochemical analysis. Soh, KL; Kang, WP; Davidson, JL; Basu, S; Wong, YM; Cliffel, DE; Bonds, AB; Swain, GM, DIAMOND AND RELATED MATERIALS, 13, 2009-2015 , (2004)

Effect of surface composition on the adsorption of photosystem I onto alkanethiolate self-assembled monolayers on gold. Ko, BS; Babcock, B; Jennings, GK; Tilden, SG; Peterson, RR; Cliffel, D; Greenbaum, E, LANGMUIR, 20, 4033-4038 , (2004)

NanoLiterBioReactor: Long-term mammalian cell culture at nanofabricated scale. Prokop, A; Prokop, Z; Schaffer, D; Kozlov, E; Wikswo, J; Cliffel, D; Baudenbacher, F, BIOMEDICAL MICRODEVICES, 6, 325-339 , (2004)

Synthesis and catalytic properties of soluble platinum nanoparticles protected by a thiol monolayer. Eklund, SE; Cliffel, DE, LANGMUIR, 20, 6012-6018 , (2004)

CVD diamond anisotropic film as electrode for electrochemical sensing. Soh, KL; Kang, WP; Davidson, JL; Wong, Y; Wisitsora-at, A; Swain, G; Cliffel, DE, SENSORS AND ACTUATORS B-CHEMICAL, 91, 39-45 , (2003)

Modification of the Cytosensor (TM) microphysiometer to simultaneously measure extracellular acidification and oxygen consumption rates. Eklund, SE; Cliffel, DE; Kozlov, E; Prokop, A; Wikswo, J; Baudenbacher, F, ANALYTICA CHIMICA ACTA, 496, 93-101 , (2003)

Boron-doped CVD diamond planar film as an electrode for electrochemical sensing. Soh, KL; Kang, WP; Davidson, JL; Wong, YM; Wisitsora-at, A; Swain, G; Cliffel, DE, PROCEEDINGS - ELECTROCHEMICAL SOCIETY, 2002-6, 235-238 , (2002)

The electrochemistry of monolayer protected Au clusters. Cliffel, DE; Hicks, JF; Templeton, AC; Murray, RW, METAL NANOPARTICLES, , 297-317 , (2002)

A Pt-Ru/graphitic carbon nanofiber nanocomposite exhibiting high relative performance as a direct-methanol fuel cell anode catalyst. Steigerwalt, ES; Deluga, GA; Cliffel, DE; Lukehart, CM, JOURNAL OF PHYSICAL CHEMISTRY B, 105, 8097-8101 , (2001)

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