October 7, 2009
FRONTIERS IN MATERIALS SCIENCE
VINSE COLLOQUIUM SERIES
Dr. Luke Lee
Lloyd Distinguished Professor, Bioengineering
Director, Biomolecular Nanotechnology Center
Co-director, Berkeley Sensor & Actuator Center
University of California, Berkeley
"Nano and Biotechnology Convergence for Precision Biology & Personalized Medicine: Satellite Nanoscopes and OASIS"Abstract. It is critical time to challenge the problems of current qualitative biology and to find effective solutions for diagnostics and therapies in 21st century medicine. In this talk, I will discuss satellite nanoscopes for spectroscopic imaging of living cells and the optical control of gene regulation and protein expression. I will also share my vision for quantitative biology and personalized medicine via *Optofluidic Application Specific Integrated Systems (OASIS). The OASIS can provide solutions for systematic biology and high-speed drug screening, and point of care molecular diagnostics. Satellite Nanoscopes To find new solutions using nature as a tool for inspiration, we have studied electron transfer mechanisms in biological systems and developed satellite nanoscopes, which can perform quantized Plasmon Resonance Energy Transfer (PRET) nanospectroscopy, gene delivery, and optical gene control in living cells. Since understanding gene function and regulation are the foundation of biology and medicine, which requires precision experiments rigorous measurements in live cells and organisms, we have developed Oligonucleotides on a Nanoplasmonic Carrier Optical Switches (ONCOS) to control gene regulation and protein expression. ONCOS allows on-demand gene silencing with nanometer-scale spatial resolution and localized temperature modulation in living cells. Nanobiophotonic molecular ruler is also accomplished to measure the dynamics of enzymes, DNA, and RNA-protein interactions.
OASIS In order to accomplish physiologically relevant cell culture and stem cell biology, we have developed Biofluidic Application Specific Integrated Systems (BioASICs) by connecting novel microfluidics and nanofluidic circuits for important biological experiments. The BioASICs can impact on high-speed and high-content precision biology, and quantitative medicine in new ways. We are creating a library of these “building blocks" to develop innovative single cell array, physiologically relevant dynamic cell culture array, and biological microprocessors with integrated optical controls and detections capability. Integrating innovative optofluidics and BioASICs in order to overcome barriers of current biological questions creates OASIS. I will discus the critical role of 21st precision biology via OASIS and the vision for predictive, preventive, and personalized medicine.
Biography. Prof. Luke P. Lee is Lloyd Distinguished Professor of Bioengineering at UC Berkeley. He is also Director of Biomolecular Nanotechnology Center and Co-Director of Berkeley Sensor & Actuator Center. He was Chair Professor in Systems Nanobiology at the Swiss Federal Institute of Technology (ETH, Zurich). He received both his B.A. and Ph.D. from UC Berkeley. His research interests are molecular imaging of living cells, single cell biophysics, systems nanobiology, molecular diagnostics, soft-matter biophysics, and Biologically-inspired Photonic-Optofluidic-Electronics Technology and Science (BioPOETS). Prof. Lee has authored and co-authored over 220 papers on biophotonics, integrated molecular diagnostics, quantitative cell biology, BioMEMS, integrated optical microfluidics, SERS, SQUIDs, and nanogap biosensor for label-free biomolecule detection.
Dr. Luke Lee