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Safety is always the primary responsibility of researchers, whether first year students or seasoned research veterans. Safety of self and others goes well beyond activities in the lab and even has far reaching implications in ethics and human rights. The potential for a researcher's work to affect humanity on a global scale, either positively or negatively, is a great responsibility. It is up to each researcher to protect humanity while still providing valuable content to the body of all human knowledge.

Safety in an interdisciplinary environment can become quite complex as people from different backgrounds work together in the same laboratory setting, exploring unfamiliar ground and setting new boundaries for science. The safety concerns of engineers are often not the same as those of biologists and vice versa. This dynamic makes knowledge of safety even more important when designing apparatus and experiments that will be used both in VIIBRE Labs and labs abroad.

Guide for handling chemicals in VIIBRE Labs

MSDS

The first thing you should always do is read the MSDS - Material Safety Data Sheet. These data sheets will give you vital information about dangers related to the chemicals you are using as well as the type of Personal Protective Equipment you should use. Your protocol designs should take into consideration the information available in the MSDS's of the chemicals used. MSDS's are available in every lab that chemicals are allowed in and there is a master library available from the lab manager. If you are introducing new chemicals to any lab you must make sure that the chemical is approved by the lab P.I. and the lab manager and that the MSDS is inserted into that lab's MSDS book. Before mixing any chemicals you MUST have an approved protocol if there is not one already.
New Protocol Form

PPE

PPE - Personal Protective Equipment - is very important, so learn how to read an MSDS to see what type of PPE you need to handle each chemical in your protocol. Remember to always wear the PPE required by the most dangerous material in use. The minimum PPE when using ANY chemical ANYWHERE on campus is safety glasses, gloves, and lab coat. Most MSDS's will not tell you what type of gloves or what type of respiratory protection you need. It is up to you to find out what types of gloves are compatible with the chemicals that you will use and to determine the best respiratory protection for your experiment. Every glove is different and you should not assume that one nitrile glove is the same as the next. You should always look at the chemical resistance data for each type and brand of glove that you use.

Chemical Storage

In most cases you will not use the entire supply of a chemical as soon as you remove it from the shipping box. This means that you will need a place to store the chemical. Chemicals stored in the same cabinet must be compatible and the cabinet must be rated to store the chemicals that you put in it. It is very important that you know where you will store your chemicals even before you order them. Some basic guidelines are: Never store acids with organics. Never store acids with bases. Never store acids with oxidizers. Never store flammables with oxidizers. Never store flammables with bases. Never store bases with oxidizers. Organics in any of the categories should be stored separate from inorganics of the same category. There are some chemicals that should be stored separate from all other chemicals and some that need to be stored under inert atmosphere. The MSDS for the chemicals will tell you how to store them, if there is any question. Under no circumstances should you ever store any chemicals at your desk.

Chemical Disposal

At the end of each experiment you are likely to have some kind of chemical waste. Vanderbilt does NOT allow ANY chemical disposal down the drains. You should put your used chemicals in an approved and chemically compatible container properly marked with the contents, fill out a pink collection tag supplied by VEH&S and notify VEH&S via their web site to pick up the waste. Make sure the chemicals you put in the container are compatible, as some chemical mixtures can generate explosive gases or may generate explosive pressure in a closed container. Make sure you are aware of the reaction possibilities of the chemicals you put in waste containers. Your experimental protocols should address proper chemical disposal.
VEH&S Guide for Managing Chemical Waste

Notes on Chemical Use

Once everything is prepared for proper handling of your chemicals, you are ready to start your experiment. Your experimental protocol should include instructions on what type of containers you should use for your chemicals during the process. There are a lot of reasons to use specific materials for experimental containers, including but not limited to process contamination, spill hazards, and adverse chemical reactions (e.g., you should not use polystyrene petri dishes to soak a substrate in acetone).

Please read the MSDS for ALL CHEMICALS used in your experiments. If you are unable to locate an MSDS for one of your chemicals, please contact Ron Reiserer. He will find it for you and make sure it is available for others to read as well. It is also a good idea to know what chemicals are being used in experiments conducted by others in the same lab space that you use. It can be difficult to identify spilled chemicals and you should be aware of potential dangers. If you spill something (even water) please clean it up immediately so others don't have to wonder what it is.

If you are ordering new chemicals you MUST get the approval of your advisor or supervisor AND you MUST have the approval from the P.I. of the lab in which it will be used. For all hazardous chemicals you must also have a written and approved protocol for all experiments in which the chemical is used. For VIIBRE labs, hazardous chemicals are any chemical with a 1 or greater number in any part of the hazard diamond or chemicals marked with the words toxic, hazardous, harmful, dangerous, flammable, explosive, corrosive, or oxidizer. If there is any doubt then it IS hazardous.

If you have any questions or concerns please contact: Ron Reiserer by campus phone 22569 or email r.reiserer@vanderbilt.edu.

Remember that NO experiment is worth your life. Don't take chances with safety!