Zeiss Axiovert 200M

Inverted wide-field fluorescence microscope equipped with a temperature controlled CO₂ incubation system for live cell time-lapse experiments.

• • Objectives: 1.25x EC Plan NEOFLUAR (NA 0.03, WD 4.0mm, 0.17mm coverglass), 10x CP-"Achromat" (NA 0.25, WD 5.1mm, 0.17mm coverglass), 20x LD A-Plan (NA 0.3, WD 4.2mm, 0-2mm coverglass), 20x LD-ACHROPLAN (NA 0.4, WD 10.2mm, 0-1.5mm coverglass w/corr.collar), 40x LD-ACHROPLAN (NA 0.6, WD 2.5mm, 0-2mm coverglass w/corr.collar), 63x Oil Plan APOCHROMAT (NA 1.4, WD 0.19mm, 0.17mm coverglass, oil immersion) 
  • Brightfield, Phase I/II/III, DIC II/III
  • Fluorescent Filters: FITC, DAPI, Texas Red, Rhodamine
  • Photometrics CoolSNAP _HQ  12-bit CCD camera
  • Motorized XY-stage, objective turret, shutters

  • Metamorph software

Nikon Eclipse Ti-E

Fully motorized inverted wide-field fluorescence microscope.

• • Objectives: 10x CFI Plan Fluor DL (NA 0.3, WD 16mm), 20x CFI Super Plan Fluor ELWD (NA 0.45, WD 8.2mm), 40x CFI Super Plan Fluor ELWD (NA 0.6, WD 3.6mm)
  • Brightfield, Phase I/II, DIC
  • Fluorescent Filters: FITC, DAPI, Texas Red
  • Photometrics CoolSNAP-HQ² 14-bit CCD camera
  • Motorized XYZ-stage (w/encoder), nosepiece, objective turret, shutters, fluorescence filters, condenser turret
  • Integrated Perfect Focus System (PFS)
  • Nikon NIS-Elements software (incl. 6D imaging module)

The Opera QEHS is available to all Vanderbilt researchers through C.O.R.E.S.!

Please visit our Automated Biosystems Core website to find out more.

PerkinElmer Opera QEHS 2.0

Highly fexlible, fully automated and fastest HCS/HTS confocal microscope at Vanderbilt.

• • Objectives: 10x Air (NA 0.4, WD 3.1mm, conf.depth 6 microns), 20x Air (NA 0.45, WD 6.9mm, conf.depth 6um, w/corr.collar), 40x Air (NA 2.7, WD 2.7mm, conf. depth 2.7um, w/corr.collar), 20x Water Immersion (NA 0.7, WD 0.6mm, conf. depth 2um), 40x Water Immersion (NA 0.9, WD 0.16mm, conf. depth 1.2um)
  • 4 laser excitation sources (405, 488, 561, 640nm), tunable UV/Vis (350-410nm), TLED (brightfield)
  • Wide range of fluorescent emission filter combinations (450/50, 520/35, 540/75, 565/40, 585/40, 600/40, 690/50)
  • 3 Peltier cooled high-res CCD cameras (1.3 MP, 12-bit), 1 non-confocal CCD camera (for UV/Vis)
  • Motorized stage, z-focus drive, water immersion
  • Accepts 96, 384, 1536-well plates, microscope slides, and custom microfluidics
  • Efficient and detailed high-volume image analysis capabilities using PerkinElmer Columbus or Volocity 3D software