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Automated
Biosystems
Core Your High Content Screening Resource
With VIIBRE's PerkinElmer Opera QEHS, you can conduct high-throughput, high-content assays of 96, 384, 1536, glass slides, or use VIIBRE's custom microfluidic wellplates. The instrument is capable of acquiring 80+ 1.3 MP, 12-bit confocal images per minute (w/ water-immersion objectives) utilizing up to 4 cooled CCD cameras, 4 lasers and tunable UV source.
The confocal spinning-disk technique with 40 ms minimum exposure times allows for minimal bleaching or your sample, while taking high-quality images at very high frame rates, which makes it ideal for live cell imaging. A multitude of optical filters and separate or combined exposures meet are wide range of fluorescent labeling needs.
C.O.R.E.S. Account Setup Instructions
As an active VIIBRE associate, you are required to use the CORES booking system to reserve time in the Automated Biosystems Core labs, first request access as an authorized user of the CORES system using the form available at http://www.mc.vanderbilt.edu/root/vumc.php?site=CFUIS&doc=13512 . Account authorization may take a few days so plan ahead.
Once you have received notification from the Office of Research that you have a CORES account, or if you are already using the CORES system, please send an email to the VIIBRE Mirofabrication Core Manager [ABCmanager@vanderbilt.edu] for final account approval and details on training and additional information. You'll then receive notification that you're ready to go, along with instructions on how to use the new booking system.
Example Applications |
Systems biology, RNAi screening
Analysis of gene expression, gene activity and protein regulation, cell signaling and pathway screening, target identification
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Toxicity testing
Cell viability, cell differentiation, proliferation, cytotoxicity, apoptosis, transporter phenomena, membrane potential, phospholipidosis, hepatosteatosis, peroxisome quantification, mitochondrial mass, nuclear fragmentation |
Stem cell screening
Reprogramming, proliferation, differentiation |
Drug discovery: Primary and secondary screening, follow-up testing
Ligand binding, receptor activation and desensitization, recruitment of signaling molecules, membrane ruffling, endocytosis, translocation, calcium flux, second messen-gers, ion channels, cell cycle |
Phenotypic Assays
Neurite outgrowth, cell differentiation, cell adhesion and spreading, cytoskeletal rearrangement, migration, invasion |
Ultra-Highspeed Confocal Screening MicroscopePerkinElmer Opera QEHS |
- 4 laser sources (405, 488, 561, 640nm
- Tunable UV/Vis (350-410nm)
- Exposure times as short as 40ms - perfect for live-cell imaging
- 4 Peltier-cooled high resolution CCD cameras (1.3 MP, 12-bit)
- Multiple options for filter selection, simultaneous or separate exposures to minimize excitation/emission overlap
- Air and water immersion objectives
- Ultra-fast laser based autofocus (0.1s)
- Motorized stage and z-focus drive
Opera User Policies
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| Columbus Imaging Software |
Key Features
- Comprehensive and intuitive software with pre-configured building blocks and semi-automated architecture leads to short learning curves, fast results
- High-volume image data analysis: Analyze complete wellplates in batches.
- Powerful machine-learning capabilities lets user train the software to develop new algorithms using a point-and-click approach
- Analysis scripts building blocks: e.g. find and segment cells by nuclei, cytoplasm, spots, micronuclei, neurites, texture, and calculate fluorescent intensity, morphology properties by channel, area, and cell parts
- Supports microscopy images from any system (e.g. Zeiss) and exports to Excel, PowerPoint
- Range of data management tools (review entire history of an experiment, explore data by various channels and metadata)
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Rates
Opera QEHS - UltraHighspeed Confocal Microscope
$60.00/hr
Columbus Imaging Software
$15.00/hr
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