DNA Sequencing Facility

The DNA Sequencing Facility uses state-of-the-art technology and methodology to produce high-quality DNA sequences in 1-2 business days. DNA samples are sequenced using Big Dye Terminator chemistry and resolved on one of two ABI 3730xl automated sequencers. Typical read lengths range from 800 to 1200 bases. Reactions are set up robotically using a PerkinElmer Janus workstation and cycled on an ABI 9800 Fast Thermal Cycler. The ABI sequencer uses capillary electrophoresis that creates a sensitive detection system, long sequence reads, short run times, and low operating/reagent costs. This automated system coupled with the facility's liquid handling robot dramatically reduces human error. The DNA Sequencing Facility is a member of the Genome Technology Core (GTC) that performs ultra-high throughput sequencing projects using an Illumina Genome Analyzer. The facility also offers high-throughput multiplexed genotyping.

Contact: Robert Woodhall—Facility Manager
Phone: 615-936-2660
Email: robert.woodhall@vanderbilt.edu
Website: seq.mc.vanderbilt.edu/DNA