Technology
Instruments
The HTS facility houses state-of-the-art liquid handlers and multi-mode plate readers that can be integrated into screening systems for automated HTS applications. The suite of plate readers contains instruments with varying sensitivities for all common modalities including absorbance, fluorescence, time-resolved fluorescence, fluorescence polarization and luminescence. Additionally, we offer label-free, alpha screen, scintillation proximity and high-content screening technologies. Liquid handling capabilities allow for volume transfers ranging from 2.5 nanoliters to liters, in various combinations of fixed and random plate arrays. Readers and liquid handlers are available for walk-up use, as well as automated screening. Furthermore, the facility staffs a full-time engineer to ensure the instrumentation meets or exceeds industry specifications for operation.
Data Analysis and Storage
The informatics and statistical capabilities of the HTS facility include data storage, archival and analysis of data, as well as custom databases and applications tailored for HTS applications. The integrity of the large volumes of data generated in the HTS facility is maintained by an infrastructure centered on redundant data storage, both at the laboratory and at a central Vanderbilt data repository with virus scans and backup preformed daily. Secured facility access and video monitoring provide for physical security of the facility whereas the data network itself is protected by a dual-layer firewall. Transfer rates are preserved by the compartmentalization of robots and controllers into a sub-network. To exceed the required capacity of the system, upgrades are liberal with the entire network retrofitted on a two-year cycle. Data processing, storage and custom informatics solutions are all offered on a fee-for-service basis and can be requested by submitting the Intake Form. |
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Microplate Readers
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| Instrument |
FDSS 6000 |
EnSpire 2300 |
Spectramax M5 |
TopCount NXT |
Envision 2100 |
| Manufacturer |
Hamamatsu |
PerkinElmer |
Molecular Devices |
PerkinElmer |
PerkinElmer |
| Type |
Whole plate kinetic imaging |
Multimodal iterative well scanning |
Multimodal iterative well scanning |
Scintillation and luminescence counter |
Multimodal iterative well scanning |
| Options |
Fluorescence, luminescence |
Fluorescence, high-sensitivity luminescence, absorbance, ALPHA, label-free |
Fluorescence, luminescence, absorbance, fluorescence polarization, time resolved-FRET |
Scintillation and Luminescence |
Fluorescence, luminescence, absorbance, fluorescence polarization, time-resolved FRET |
| Other Info |
384 well plates |
24, 48, 96, or 384-well plates |
6,12,24,48,96, or 384-well plates, cuvettes |
24, 96, or 384-well plates |
24, 96, 384, 1536-well plates |
Thermo CRS robotics, Cytomat incubators, and barcode printers are available with automation. Plate sealers, centrifuges, and shakers are available as peripheral equipment. |
Liquid Handling
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| Instrument |
Echo 550/555 |
Bravo |
VPrep |
Multidrop Combi |
ELx405 |
| Manufacturer |
Labcyte |
Velocity 11/Agilent |
Velocity 11/Agilent |
Thermo |
BioTek |
| Type |
Automated Acoustic Liquid Transfer |
Automated Pipette Liquid Transfer |
Automated Pipette Liquid Transfer |
Liquid Dispenser |
Liquid Aspirator/Dispenser |
| Options |
DMSO soluble compounds Plate stamping, cherry-picking, dose response titrations Volumes: 2.5 - 1000 nL |
Any liquid viscosity plate stamping and serial dilutions (row or column), tip washing Volumes: 0.1 - 300 uL |
Any liquid viscosity Plate stamping Volumes: 0.1 - 300 uL |
Variable liquid viscosities High-speed 8-channel liquid dispenser using a peristaltic pump Volumes: 0.5 – 2500 uL |
Variable liquid viscosities Plate washer- 96 well manifold Volumes: 50 – 3000 uL |
| Other Info |
96, 384, or 1536-well plates |
96, 384, 1536 -well plates |
96, 384, 1536 -well plates |
96, 384, 1536 -well plates |
96 or 384-well plates |
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