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Plasmid Based Reverse Genetics for Reovirus
Reoviruses, dsRNA viruses, are a versatile experimental system for studying viral replication events at the virus-cell interface. They are particularly useful in studying activation of the innate immune response as well as internalization and disassembly. Reverse genetics systems exist for most major groups of animal RNA viruses. However, previously, there were no known systems for obtaining an animal dsRNA virus entirely from cloned cDNAs.
A novel system of generating an infectious reoviridae particle entirely from cloned cDNAs permits selective introduction and recovery of desired mutations in each viral gene segment. Such a system implements well-known techniques and can be followed by purification.
Scientists at Vanderbilt have developed an entirely plasmid-based system to better utilize reoviruses as a research tool. The system allows for generation of a reovirus entirely from cloned cDNAs comprising three steps involving fairly well-known techniques.
• Provides a system that allows for focused and rapid genetic analysis of viral phenotypes such as virus structure, virus-cell interactions, and viral pathogenesis.
• Provides a system that permits selective introduction of desired mutations into cloned cDNAs encoding the viral gene segments, followed by isolation of mutant viruses from transfected cells.
• Provides a system with potential as a gene delivery vehicle for vaccines, oncolytics, and gene-transfer vectors for research.
• Provides a platform for development of analogous marker rescue systems for other segmented dsRNA viruses
Intellectual Property Status:
U.S. Issued Patent 8,293,498; For further reading see Dermody et al. 2010.
Inventors:Terence DermodyTakeshi KobayashiJames Chappell