These cell lines are useful for studying the regulation of tissue-specific gene expression, and may also be used to identify epididymal-specific transcription factors involved in expression of specific proteins in the epididymis.
KCC2b (Slc12a5) global knockout heterozygous mice on a C57BL/6J background. The Slc12a5 gene has alternative promoters which produce isoforms starting with exon 1a (5-10% total expression) and exon 1b (90-95% expression). As we targeted exon 1b, the mouse in not a global KO of KCC2 but a hypomorphic mouse, still expressing 5-10% protein. It is a global KCC2b KO mouse. Heterozygous animals develop spontaneous seizures in cages. This increases with age. Their brain is hyperexcitable. Homozygous animals die between P17 due to repeated tonic-cloning seizures.
This technology consists of a method to fabricate a truly 2 dimensional porous surface using graphene with stabilized pore diameters less than a few nanometers. The nanopores are inert and stable for extended periods of time (several months) and under extreme conditions. The resulting membrane can be used in water purification, chemical separation, sensing, DNA sequencing, and other applications.
Kinesins are motor proteins in eukaryotic cells powered by ATP hydrolysis. These proteins are involved in various cellular functions including cell division. In particular, Kinesin-5 (also known as KIF11 and Eg5) is essential to forming the microtubule spindle structure in mitosis; therefore, this protein is a potential target for chemotherapeutics. Chimeric kinesin proteins, comprising one or more regions from at least two kinesin proteins, are valuable tools to study the molecular mechanism of kinesin function as well as to identify agents that affect kinesin motor function.