KCC2b (Slc12a5) global knockout heterozygous mice on a C57BL/6J background. The Slc12a5 gene has alternative promoters which produce isoforms starting with exon 1a (5-10% total expression) and exon 1b (90-95% expression). As we targeted exon 1b, the mouse in not a global KO of KCC2 but a hypomorphic mouse, still expressing 5-10% protein. It is a global KCC2b KO mouse. Heterozygous animals develop spontaneous seizures in cages. This increases with age. Their brain is hyperexcitable. Homozygous animals die between P17 due to repeated tonic-cloning seizures.
Kinesins are motor proteins in eukaryotic cells powered by ATP hydrolysis. These proteins are involved in various cellular functions including cell division. In particular, Kinesin-5 (also known as KIF11 and Eg5) is essential to forming the microtubule spindle structure in mitosis; therefore, this protein is a potential target for chemotherapeutics. Chimeric kinesin proteins, comprising one or more regions from at least two kinesin proteins, are valuable tools to study the molecular mechanism of kinesin function as well as to identify agents that affect kinesin motor function.
TagDock is an efficient rigid body molecular docking algorithm that generates three-dimensional models of oligomeric biomolecular complexes in instances where there is limited experimental restraint data to guide the docking calculations. Through distance difference analysis TagDock additionally recommends followup experiments to further discriminate divergent (score-degenerate) clusters of TagDock's initial solution models
Dr. Patrick Page-McCaw has developed synthetic analogs of N-acetylgalactosamine, finally enabling researchers to track the biosynthesis of chondroitin sulfate along with other glycans. These stunning images demonstrate incorporation of these metabolic labeling reagents to track neurodevelopmental processes in a zebrafish model system. Notably, the metabolic label can be detected post vivo using a standard "click" chemistry reaction. Further, Dr. Page-McCaw has optimized a background reduction strategy to complement this technology by improving the signal-to-noise ratio.