Dr. Piston's group has generated a variant of the enhanced cyan fluorescent protein (ECFP) that has improved brightness, more resistance to photo-bleaching and a fluorescence lifetime that is best fit by a single exponential.
A method to assess oxidative stress in vivo includes the steps of measuring an amount of neuroprostanes in a biological sample before the ex vivo development of neuroprostanes in a sample, comparing the measured amount of neuroprostanes with a control and assessing oxidative stress in vivo based on this comparison. There is also provided a marker for oxidated stress by an increase of neuroprostanes in a biological sample compared to a control sample. A diagnostic tool for determining the presence of a neurodegenerative disease provides for determining an increased amount of neuroprostanes in a biological sample compared to that of a control sample.
Dr. Hawiger and colleagues developed a novel form of cell-penetrating fetal hemoglobin. This technology is useful for the treatment of hemoglobinopathies or to increase oxygen delivery in any condition. The preparation is stable and sterile. Upon reconstitution, the treatment can be delivered I.V. or through transfusion.
D-3-Hydroxybutyrate (3-HB) cell culture medium is useful for studying cancer cells lines in a novel, but physiologically relevant, metabolic context. Cancer cells are known to have rapid glucose metaobolism and most cell-based research is carried out in glucose containing medium. Cancer cells alternatively use 3-HB as a carbon source, which is a prevalent metabolic substrate when fasting. 3-HB medium offers researchers the chance to study cell growth and chemotherapuetic agents in this novel and frequently overlooked context.
This technology describes a novel methodology wherein human-induced pluripotent stem cells can be differentiated into neural precuror cells using DMH1 (a dorsomorphin analog). Neural progenitor cells can then be further differentiated into tyrosine hydroxylase expressing neurons.
The mouse embryonic stem cell line, CGR8, has been genetically modified to express luciferase under the control of either the wnt or the BMP promoter system. This technology allows rapid visualization or genetic modification of wnt and BMP pathways in development.
Transforming growth factor (TGF) beta type II receptor antibodies directed towards the extracellular domain (epitope 4-19) are available from the laboratory of Harold Moses. TGF-beta-II plays an important role in several biological processes including developmental biology, cardiovascular biology and cancer.