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109 available technologies

Mouse Fc-FGF-1

We produced a plasmid containing the Fc portion of mouse IgGl (Fc) coupled to human fibroblast growth factor 1 (FGF-1). The plasmid was transformed into E. coli to express the fusion protein. The fusion protein was purified on a heparin sepharose column which has high affinity for the FGF portion of the fusion protein. The purpose of making this protein was to be able to identify cells that express receptors for FGF using flow cytometry.There are multiple fluorochrome labeled antibodies to mouse IgGl. When the fusion protein is bound to FGF receptors on cells, the Fc portion is on the surface of the cells and can be detected by fluorochrome labeled antibodies to mouse IgGl. Therefore, cells that express FGF receptors and bind the fusion protein can be detected by flow cytometry or immunofluorescence.

Cell Line with Stable Expression of Recombinant Human Chloride Channel, hCIC-4

The invention is a cell line (Human embryonic kidney 293) stably expressing a recombinant human chloride channel (hClC-4). The cells enable high throughput screening of compounds that modulate chloride channel activity.

Cell Line with Stable Expression of Human Skeletal Muscle Sodium Channel (hSkM1)

The invention is a cell line (Human embryonic kidney 293) stably expressing a recombinant human skeletal muscle voltage-gated sodium channel (hSkMl). The cells enable high throughput screening of compounds that modulate sodium channel activity.

Cell Line with Stable Expression of Recombinant Human Skeletal Muscle Chloride Channel (hCIC-1)

The invention is a cell line (Human embryonic kidney 293) stably expressing a recombinant human skeletal muscle chloride channel (hClC-1). The cells enable high throughput screening of compounds that modulate chloride channel activity.

Methods and Compositions to Assess Oxidative Brain Injury

A method to assess oxidative stress in vivo includes the steps of measuring an amount of neuroprostanes in a biological sample before the ex vivo development of neuroprostanes in a sample, comparing the measured amount of neuroprostanes with a control and assessing oxidative stress in vivo based on this comparison. There is also provided a marker for oxidated stress by an increase of neuroprostanes in a biological sample compared to a control sample. A diagnostic tool for determining the presence of a neurodegenerative disease provides for determining an increased amount of neuroprostanes in a biological sample compared to that of a control sample.

Generation of Neuroprogenitor Cells and Neurons from Human Induced Pluripotent Stem Cells by the Dorsomorphin Analog DMH1

This technology describes a novel methodology wherein human-induced pluripotent stem cells can be differentiated into neural precuror cells using DMH1 (a dorsomorphin analog). Neural progenitor cells can then be further differentiated into tyrosine hydroxylase expressing neurons.

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