Nucleic Acid/Plasmid

Expression plasmids for rat glucokinase

This research targets GK.
Licensing manager: 
Karen Rufus
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Expression vector for Leukocyte-Type 12-Lipoxygenase

This research targets 12-Lox.
Licensing manager: 
Mike Villalobos
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Mouse Focal Adhesion Kinase cDNA

This research targets FAK.
Licensing manager: 
Jody Hankins
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Prostaglandin E receptor-2 cDNA and Prostaglandin E receptor-4 cDNA

This research targets EP2, EP4.
Licensing manager: 
Karen Rufus
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Mouse cDNA for CrK-associated substrate

This research targets Cas.
Licensing manager: 
Jody Hankins
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Mouse ES Stem Cell Line Marking Cardiomyocytes

This is a genetically engineered mouse ES stem cell line marking cardiomyocytes with red fluorescent protein (RFP). These cells have been used in high-throughput screens by Vanderbilt researchers for pro-cardiogenic factors. In addition the construct used in this cell line could be used to mark the cardiac progeny of transplanted stem cells, generate stable human ES cell lines, or engineer iPS for cardiac lineage studies.
This is a genetically engineered mouse ES stem cell line marking cardiomyocytes with red fluorescent protein (RFP). These cells have been used in high-throughput screens by Vanderbilt researchers for pro-cardiogenic factors.
Licensing manager: 
Mike Villalobos
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Mouse Fc-FGF-1

We produced a plasmid containing the Fc portion of mouse IgGl (Fc) coupled to human fibroblast growth factor 1 (FGF-1). The plasmid was transformed into E. coli to express the fusion protein. The fusion protein was purified on a heparin sepharose column which has high affinity for the FGF portion of the fusion protein. The purpose of making this protein was to be able to identify cells that express receptors for FGF using flow cytometry.There are multiple fluorochrome labeled antibodies to mouse IgGl. When the fusion protein is bound to FGF receptors on cells, the Fc portion is on the surface of the cells and can be detected by fluorochrome labeled antibodies to mouse IgGl. Therefore, cells that express FGF receptors and bind the fusion protein can be detected by flow cytometry or immunofluorescence.
We produced a plasmid containing the Fc portion of mouse IgGl (Fc) coupled to human fibroblast growth factor 1 (FGF-1). The plasmid was transformed into E. coli to express the fusion protein. The fusion protein was purified on a heparin sepharose column which has high affinity for the FGF portion of the fusion protein.
Licensing manager: 
Karen Rufus
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New Gene Involved in Male Fertility

It is estimated that approximately 30% of men have reduced fertility and 2% are totally infertile. Despite these large numbers relatively little is know about the molecular bases of male infertility. On the flip side of male infertility is the need for male contraception. Currently there are no reversible, convenient male contraceptives available. In order to develop male contraceptives and acquire a greater understanding of male fertility there is a need to develop animal models to study the molecular basis and pathways that regulate and control male fertility. Vanderbilt researchers have developed a model mouse system to study male fertility. There research focuses on the epididymus, which is the area that spermatozoa acquire the ability to move and fertilize. For this region to be functional tissue and cell specific gene regulation must occur. These investigators have discovered one such gene regulated within this area, mEP17. These researchers can fuse either mouse or human EP17 or just the regulatory regions of either EP17 to reporter genes and the resulting fusion can be used to screen for substances that regulate this gene and affect male fertility. This system becomes a powerful tool to identify drugs which affect this gene and be potential male contraceptives. In addition polypeptides generated to this gene may be used as vaccines for male contraceptives.
It is estimated that approximately 30% of men have reduced fertility and 2% are totally infertile. Despite these large numbers relatively little is know about the molecular bases of male infertility. On the flip side of male infertility is the need for male contraception. Currently there are no reversible, convenient male contraceptives available.
Licensing manager: 
Jody Hankins
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Lipoxygenase Proteins and Nucleic Acids

Isolated and purified lipoxygenase proteins and nucleic acids are described. Particularly, a novel human 15(S) lipoxygenase (15-Lox-2) protein and cDNA and a cDNA for mouse 8S-lipoxygenase are described. Recombinant host cells, recombinant nucleic acids and recombinant proteins are also described, along with methods of producing each. Isolated and purified antibodies to 15-Lox-2 and 8-Lox, and methods of producing the same, are also described.
Isolated and purified lipoxygenase proteins and nucleic acids are described. Particularly, a novel human 15(S) lipoxygenase (15-Lox-2) protein and cDNA and a cDNA for mouse 8S-lipoxygenase are described. Recombinant host cells, recombinant nucleic acids and recombinant proteins are also described, along with methods of producing each.
Licensing manager: 
Janis Elsner
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