Associate Professor
Ph.D., University of Texas at Austin, 1998
d.cliffel@vanderbilt.edu
Cliffel Research Group

Multianalyte Microphysiometry
Analytical chemistry often leads the forefront of scientific discovery, as new instrumentation allows us to perform new experiments. Our group has created multianalyte microphysiometry by combining microfluidic technologies with electrochemical detection to study the dynamic behaviors of metabolic pathways. We are developing new multianalyte microphysiometry methods with applications in cancer, diabetes, and toxicology. We have added electrochemical detectors for many metabolic analytes into the microfluidic chamber to give a complete dynamical picture of the live cell population.

Nanoparticle Biomimics
The ability to mimic biomolecular recognition requires the generation of nanoscale structures that faithfully reproduce the lock & key motif of protein binding. We have designed biomimics that present targeted linear and loop peptide structures on the surface of monolayer protected gold nanoparticles. These biomimics are useful for calibrating immunoassays, especially in our immunosensor work with the quartz crystal microbalance.

Electrochemistry on the Nanoscale
Electron transfer in nanometer-size chemical systems is an important process for the creation of macromolecular electronics. We are interested in the electrochemistry of metallic nanoparticles, redox proteins like Photosystem I, and other electroactive nanomaterials that bridge the gap between bulk solids and discrete molecules in the development of electronic devices using nanotechnology.

Selected Publications

Eklund SE, Thompson RG, Snider RM, Carney CK, Wright DW, Wikswo J, Cliffel DE. Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer. Sensors. 2009, 9 (3): 2117-2133.

Rutledge RD, Huffman BJ, Cliffel DE, Wright DW. Design and synthesis of an antigenic mimic of the Ebola glycoprotein. Journal of Materials Research. 2008, 23 (12): 3161-3168.

Ciesielski PN, Scott AM, Faulkner CJ, Berron BJ, Cliffel DE, Jennings GK. Functionalized Nanoporous Gold Leaf Electrode Films for the Immobilization of Photosystem I. ACS NANO. 2008, 2 (12): 2465-2472.

Lehner S, Ciobanu M, Savage K, Cliffel DE. Electrochemical impedance spectroscopy of synthetic pyrite doped with As, Co, and Ni. Journal of The Electrochemical Society. 2008, 155 (5): P61-P70.

Soh KL, Kang WP, Davidson JL, Wong YM, Cliffel DE, Swain GM. Diamond-derived ultramicroelectrodes designed for electrochemical analysis and bioanalyte sensing. Diamond and Related Materials. 2008, 17 (4-5): 900-905.

Faulkner CJ, Lees S, Ciesielski PN, Cliffel DE, Jennings GK. Rapid assembly of photosystem I monolayers on gold electrodes. Langmuir. 2008, 24 (16): 8409-8412.

Soh KL, Kang WP, Davidson JL, Wong YM, Cliffel DE, Swain GM. Ordered array of diamond ultramicroband electrodes for electrochemical analysis. Diamond and Related Materials. 2008, 17 (3): 240-246.

Snider RM, Ciobanu M, Rue A, Cliffel DE. A multiwalled carbon nanotube/dihydropyran composite film electrode for insulin detection in a microphysiometer chamber. Analytical Chimica Acta. 2008, 609 (1): 44-52.

Ciobanu M, Taylor DE, Wilburn J, Cliffel DE. Glucose and Lactate Biosensors for Scanning Electrochemical Microscopy Imaging of Single Live Cells. Analytical Chemistry. 2008, 80 (8): 2717-2727.

Lehner S, Savage K, Ciobanu M, Cliffel DE. The effect of As, Co, and Ni impurities on pyrite oxidation kinetics: An electrochemical study of synthetic pyrite. Geochimica Et Cosmochimica Acta. 2007, 71 (10): 2491-2509.

Wilburn JP, Brown KL, Cliffel DE. Mercury-free analysis of lead in drinking water by anodic stripping square wave voltammetry. Journal of Chemical Education. 2007, 84 (2): 312-314.

Ciobanu M, Kincaid HA, Lo V, Dukes AD, Jennings GK, Cliffel DE. Electrochemistry and photoelectrochemistry of photosystem I adsorbed on hydroxyl-terminated monolayers. Journal of Electroanalytical Chemistry. 2007, 599 (1): 72-78.

Gies AP, Hercules DM, Gerdon AE, Cliffel DE. Electrospray mass spectrometry study of tiopronin monolayer-protected gold nanoclusters. Journal of the American Chemical Society. 2007, 129 (5): 1095-1104.

Wikswo JP, Prokop A, Baudenbacher F, Cliffel D, Csukas B, Velkovsky M. Engineering challenges of BioNEMS: the integration of microfluidics, micro- and nanodevices, models and external control for systems biology. Iee Proceedings-Nanobiotechnology. 2006, 153 (4): 81-101.

Yu JS, Liao HX, Gerdon AE, Huffman B, Scearce RM, McAdams M, Alam SM, Popernack PM, Sullivan NJ, Wright D, Cliffel DE, Nabel GJ, Haynes BF. Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. Journal of Virological Methods. 2006, 137 (2): 219-228.

Gerdon AE, Wright DW, Cliffel DE. Epitope mapping of the protective antigen of B-anthracis by using nanoclusters presenting conformational peptide epitopes. Angewandte Chemie-International Edition. 2006, 45 (4): 594-598.

Wilburn JP, Wright DW, Cliffel DE. Imaging of voltage-gated alamethicin pores in a reconstituted bilayer lipid membrane via scanning electrochemical microscopy. Analyst. 2006, 131 (2): 311-316.

Eklund SE, Snider RM, Wikswo J, Baudenbacher F, Prokop A, Cliffel DE. Multianalyte microphysiometry as a tool in metabolomics and systems biology. Journal of Electroanalytical Chemistry. 2006, 587 (2): 333-339.

Gerdon AE, Wright DW, Cliffel DE. Hemagglutinin linear epitope presentation on monolayer-protected clusters elicits strong antibody binding. Biomacromolecules. 2005, 6 (6): 3419-3424.

Peterson RR, Cliffel DE. Continuous free-flow electrophoresis of water-soluble monolayer-protected clusters. Analytical Chemistry. 2005, 77 (14): 4348-4353.

Gerdon, AE, Wright, DW, Cliffel, DE. Quartz Crystal Microbalance Detection of Glutathione-Protected Nanoclusters Using Antibody Recognition. Analytical Chemistry. 2005, 77 (1): 304-310.

Ciobanu, M, Kincaid, HA, Jennings, GK, Cliffel, DE. Photosystem I Patterning Imaged by Scanning Electrochemical Microscopy. Langmuir. 2005, 21 (2): 692-698.

Eklund SE, Taylor D, Kozlov E, Cliffel DE. A Microphysiometer for Simultaneous Measurement of Changes in Extracellular Glucose, Lactate, Oxygen, and Acidification Rate. Analytical Chemistry. 2004, 76 (3): 519-527.

Eklund SE, Cliffel DE. Synthesis and Catalytic Properties of Soluble Platinum Nanoparticles Protected by a Thiol Monolayer. Langmuir. 2004, 20 (14): 6012-6018.

Eklund SE, Cliffel DE, Kozlov E. Modification of the Cytosensor (TM) Microphysiometer to Simultaneously Measure Extracellular Acidification and Oxygen Consumption Rates. Analytica Chimica Acta. 2003, 496 (1-2): 93-101.

Specialties

• Nanoparticle Biomimics
• Electronanalytical Chemistry
• Bioanalytical Chemistry

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